Ca2+-induced Ca2+ release in the pancreatic β-cell:: Direct evidence of endoplasmic reticulum Ca2+ release

被引：39

作者：

Graves, TK ^{[1
]}

Hinkle, PM ^{[1
]}

机构：

[1] Univ Rochester, Med Ctr, Dept Physiol & Pharmacol, Rochester, NY 14642 USA

来源：

ENDOCRINOLOGY | 2003年 / 144卷 / 08期

关键词：

D O I：

10.1210/en.2002-0104

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

The role of the Ca2+-induced Ca2+ release channel (ryanodine receptor) in MIN6 pancreatic beta-cells was investigated. An endoplasmic reticulum (ER)-targeted "cameleon" was used to report lumenal free Ca2+. Depolarization of MIN6 cells with KCl led to release of Ca2+ from the ER. This ER Ca2+ release was mimicked by treatment with the ryanodine receptor agonists caffeine and 4-chloro-m-cresol, reversed by voltage-gated Ca2+ channel antagonists and blocked by treatment with antagonistic concentrations of ryanodine. The depolarization-induced rise in cytoplasmic Ca2+ was also inhibited by ryanodine, which did not alter voltage-gated Ca2+ channel activation. Both ER and cytoplasmic Ca2+ changes induced by depolarization occurred in a dose-dependent manner. Glucose caused a delayed rise in cytoplasmic Ca2+ but no detectable change in ER Ca2+. Carbamyl choline caused ER Ca2+ release, a response that was not altered by ryanodine. Taken together, these results provide strong evidence that Ca2+-induced Ca2+ release augments cytoplasmic Ca2+ signals in pancreatic beta-cells.

引用

页码：3565 / 3574

页数：10

共 45 条

[11] RAPID IONIC MODIFICATIONS DURING THE AEQUORIN-DETECTED CALCIUM TRANSIENT IN A SKINNED CANINE CARDIAC PURKINJE-CELL [J].

FABIATO, A .

JOURNAL OF GENERAL PHYSIOLOGY, 1985, 85 (02) :189-246

[12] Caffeine releases a glucose-primed endoplasmic reticulum Ca2+ pool in the insulin secreting cell line INS-1 [J].

Gamberucci, A ;

Fulceri, R ;

Pralong, W ;

Bánhegyi, G ;

Marcolongo, P ;

Watkins, SL ;

Benedetti, A .

FEBS LETTERS, 1999, 446 (2-3) :309-312

[13] Non-hyperbolic calcium calibration curve of Fura-2: Implications for the reliability of quantitative Ca2+ measurements [J].

Henke, W ;

Cetinsoy, C ;

Jung, K ;

Loening, S .

CELL CALCIUM, 1996, 20 (03) :287-292

[14] Triggering and amplifying pathways of regulation of insulin secretion by glucose [J].

Henquin, JC .

DIABETES, 2000, 49 (11) :1751-1760

[15] cAMP-dependent mobilization of intracellular Ca2+ stores by activation of ryanodine receptors in pancreatic β-cells -: A Ca2+ signaling system stimulated by the insulinotropic hormone glucagon-like peptide-1-(7-37) [J].

Holz, GG ;

Leech, CA ;

Heller, RS ;

Castonguay, M ;

Habener, JF .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (20) :14147-14156

[16] Ionic selectivity of low-affinity ratiometric calcium indicators: mag-Fura-2, Fura-2FF and BTC [J].

Hyrc, KB ;

Bownik, JM ;

Goldberg, MP .

CELL CALCIUM, 2000, 27 (02) :75-86

[17]

IMAI A, 1987, METHOD ENZYMOL, V141, P100

[18] The ryanodine receptor calcium channel of β-cells -: Molecular regulation and physiological significance [J].

Islam, MS .

DIABETES, 2002, 51 (05) :1299-1309

[19] In situ activation of the type 2 ryanodine receptor in pancreatic beta cells requires cAMP-dependent phosphorylation [J].

Islam, MS ;

Leibiger, I ;

Leibiger, B ;

Rossi, D ;

Sorrentino, V ;

Ekström, TJ ;

Westerblad, H ;

Andrade, FH ;

Berggren, PO .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (11) :6145-6150

[20] Amplification of exocytosis by Ca 2+-induced Ca2+ release in INS-1 pancreatic β cells [J].

Kang, GX ;

Holz, GG .

JOURNAL OF PHYSIOLOGY-LONDON, 2003, 546 (01) :175-189

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