MicroRNAs Play a Critical Role in Tooth Development

被引：105

作者：

Cao, H. ^{[1
]}

Wang, J. ^{[1
]}

Li, X. ^{[1
]}

Florez, S. ^{[1
]}

Huang, Z. ^{[1
]}

Venugopalan, S. R. ^{[1
]}

Elangovan, S. ^{[2
]}

Skobe, Z. ^{[2
]}

Margolis, H. C. ^{[2
]}

Martin, J. F. ^{[1
]}

Amendt, B. A. ^{[1
]}

机构：

[1] Texas A&M Hlth Sci Ctr, Inst Biosci & Technol, Houston, TX 77030 USA

[2] Forsyth Inst, Dept Biomineralizat, Boston, MA USA

来源：

JOURNAL OF DENTAL RESEARCH | 2010年 / 89卷 / 08期

关键词：

Dicer1; dental stem cells; craniofacial and tooth microRNAs; epithelial differentiation; STEM-CELLS; DENTAL EPITHELIUM; MOUSE; DIFFERENTIATION; AMELOBLASTIN; PITX2; DICER;

D O I：

10.1177/0022034510369304

中图分类号：

R78 [口腔科学];

学科分类号：

100302 [口腔临床医学];

摘要：

MicroRNAs are known to regulate gene function in many tissues and organs, but their expression and function, if any, in tooth development are elusive. We sought to identify them by microRNA screening analyses and reveal their overall roles by inactivating Dicer1 in the dental epithelium and mesenchyme. Discrete sets of microRNAs are expressed in molars compared with incisors as well as epithelium compared with mesenchyme. Conditional knockout (cKO) of Dicer1 (mature microRNAs) in the dental epithelium of the Pitx2-Cre mouse results in multiple and branched enamel-free incisors and cuspless molars, and change in incisor patterning and in incisor and molar size and shape. Analyses of differentiating dental epithelial markers reveal a defect in ameloblast differentiation. Conversely, the cervical loop (stem cell niche) is expanded in Dicer1 cKO. These results demonstrate that tooth development is tightly controlled by microRNAs and that specific microRNAs regulate tooth epithelial stem cell differentiation.

引用

页码：779 / 784

页数：6

共 26 条

[1]

MicroRNAs: Genomics, biogenesis, mechanism, and function (Reprinted from Cell, vol 116, pg 281-297, 2004) [J].

Bartel, David P. .

CELL, 2007, 131 (04) :11-29

[2]

Dicer is essential for mouse development [J].

Bernstein, E ;

Kim, SY ;

Carmell, MA ;

Murchison, EP ;

Alcorn, H ;

Li, MZ ;

Mills, AA ;

Elledge, SJ ;

Anderson, KV ;

Hannon, GJ .

NATURE GENETICS, 2003, 35 (03) :215-217

[3]

MicroRNAs modulate hematopoietic lineage differentiation [J].

Chen, CZ ;

Li, L ;

Lodish, HF ;

Bartel, DP .

SCIENCE, 2004, 303 (5654) :83-86

[4]

Modification of gene activity in mouse embryos in utero by a tamoxifen-inducible form of Cre recombinase [J].

Danielian, PS ;

Muccino, D ;

Rowitch, DH ;

Michael, SK ;

McMahon, AP .

CURRENT BIOLOGY, 1998, 8 (24) :1323-1326

[5]

Mouse models of tooth abnormalities [J].

Fleischmannova, Jana ;

Matalova, Eva ;

Tucker, Abigail S. ;

Sharpe, Paul T. .

EUROPEAN JOURNAL OF ORAL SCIENCES, 2008, 116 (01) :1-10

[6]

Ameloblastin is a cell adhesion molecule required for maintaining the differentiation state of ameloblasts [J].

Fukumoto, S ;

Kiba, T ;

Hall, B ;

Iehara, N ;

Nakamura, T ;

Longenecker, G ;

Krebsbach, PH ;

Nanci, A ;

Kulkarni, AB ;

Yamada, Y .

JOURNAL OF CELL BIOLOGY, 2004, 167 (05) :973-983

[7]

Shh signaling within the dental epithelium is necessary for cell proliferation, growth and polarization [J].

Gritli-Linde, A ;

Bei, M ;

Maas, R ;

Zhang, XYM ;

Linde, A ;

McMahon, AP .

DEVELOPMENT, 2002, 129 (23) :5323-5337

[8]

Localization of putative stem cells in dental epithelium and their association with notch and FGF signaling [J].

Harada, H ;

Kettunen, P ;

Jung, HS ;

Mustonen, T ;

Wang, YA ;

Thesleff, I .

JOURNAL OF CELL BIOLOGY, 1999, 147 (01) :105-120

[9]

New perspectives on tooth development and the dental stem cell niche [J].

Harada, H ;

Ohshima, H .

ARCHIVES OF HISTOLOGY AND CYTOLOGY, 2004, 67 (01) :1-11

[10]

Micrornas: Small RNAs with a big role in gene regulation [J].

He, L ;

Hannon, GJ .

NATURE REVIEWS GENETICS, 2004, 5 (07) :522-531

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