T-Wave morphology parameters based on principal component analysis reproducibility and dependence on T-Offset position

Background: T-wave morphology parameters based on principal component analysis (PCA) are candidate to better understand the relation between QT prolongation and torsades de pointes. We aimed to assess the repeatability and to determine the influence of T-end position on PCA parameters. Methods: Digital ECGs recorded from 30 subjects were used to assess short term (5 minutes), circadian and long-term (28 days) repeatability of PCA parameters. The T-end cursor position was moved backward and forward ( +/- 8 ms) from its optimal position. We calculated QRS-T angle, PCA ratio, and T-wave residuum (TWR). Results: At long-term evaluation, coefficients of variation were 11.3 +/- 9.9%, 11.7 +/- 7.1%, and 23.0 +/- 22.0% for the QRS-T angle, PCA ratio, TWR, respectively. After moving the T-end cursor, repeatability was 0.42 +/- 10.2%, 1.00 +/- 1.04%, 4.0 +/- 4.2% for the same PCA parameters. Conclusions: T-wave morphology parameters based on PCA are reproducible with the exception of TWR and QRS-T angle. In addition, PCA is robust, showing only little dependence on T-end cursor position. These data should be taken into account for safety pharmacology trials.