CRISPR/Cas9 Platforms for Genome Editing in Plants: Developments and Applications

被引：296

作者：

Ma, Xingliang ^{[1
,2
,3
]}

Zhu, Qinlong ^{[1
,2
,3
]}

Chen, Yuanling ^{[1
,2
,3
]}

Liu, Yao-Guang ^{[1
,2
,3
]}

机构：

[1] State Key Lab Conservat & Utilizat Subtrop Agrobi, Guangzhou 510642, Guangdong, Peoples R China

[2] Guangdong Prov Higher Educ Inst, Key Lab Plant Funct Genom & Biotechnol, Guangzhou 510642, Guangdong, Peoples R China

[3] South China Agr Univ, Coll Life Sci, Guangzhou 510642, Guangdong, Peoples R China

来源：

MOLECULAR PLANT | 2016年 / 9卷 / 07期

关键词：

CRISPR/Cas9; genome editing; multiplex editing; mutation; plants; AGROBACTERIUM-MEDIATED TRANSFORMATION; RNA-GUIDED ENDONUCLEASE; DOUBLE-STRAND BREAKS; TARGETED MUTAGENESIS; HOMOLOGOUS RECOMBINATION; CRISPR-CAS9; NUCLEASES; DIRECTED MUTAGENESIS; GENE MODIFICATIONS; DNA-RECOGNITION; HUMAN-CELLS;

D O I：

10.1016/j.molp.2016.04.009

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The clustered regularly interspaced short palindromic repeat (CRISPR)-associated protein9 (Cas9) genome editing system (CRISPR/Cas9) is adapted from the prokaryotic type II adaptive immunity system. The CRISPR/Cas9 tool surpasses other programmable nucleases, such as ZFNs and TALENs, for its simplicity and high efficiency. Various plant-specific CRISPR/Cas9 vector systems have been established for adaption of this technology to many plant species. In this review, we present an overview of current advances on applications of this technology in plants, emphasizing general considerations for establishment of CRISPR/Cas9 vector platforms, strategies for multiplex editing, methods for analyzing the induced mutations, factors affecting editing efficiency and specificity, and features of the induced mutations and applications of the CRISPR/Cas9 systemin plants. In addition, we provide a perspective on the challenges of CRISPR/Cas9 technology and its significance for basic plant research and crop genetic improvement.

引用

页码：961 / 974

页数：14

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