Complex Regulation of Two Target Genes Encoding SPX-MFS Proteins by Rice miR827 in Response to Phosphate Starvation

被引:171
作者
Lin, Shu-I [1 ]
Santi, Carole [2 ]
Jobet, Edouard [2 ]
Lacut, Elodie
El Kholti, Naima [3 ]
Karlowski, Wojciech M. [4 ]
Verdeil, Jean-Luc [3 ]
Breitler, Jean Christophe [3 ]
Perin, Christophe [3 ]
Ko, Swee-Suak [1 ,5 ]
Guiderdoni, Emmanuel [3 ]
Chiou, Tzyy-Jen [1 ]
Echeverria, Manuel [2 ]
机构
[1] Acad Sinica, Agr Biotechnol Res Ctr, Taipei 115, Taiwan
[2] Univ Perpignan, CNRS, UMR5096, Lab Genome & Dev Plantes,IRD, F-66860 Perpignan, France
[3] Univ Montpellier 2, DAP, UMR 1098, CIRAD,INRA,SUPAGRO, F-34398 Montpellier 5, France
[4] Adam Mickiewicz Univ, Bioinformat Lab, PL-61614 Poznan, Poland
[5] Acad Sinica, Biotechnol Ctr So Taiwan, Tainan 744, Taiwan
关键词
MFS domain; MicroRNA; Phosphate; SPX proteins; SMALL RNAS; PLANT-RESPONSES; ARABIDOPSIS; MICRORNAS; HOMEOSTASIS; EXPRESSION; TRANSPORT; DOMAIN; PHO2; IDENTIFICATION;
D O I
10.1093/pcp/pcq170
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Here we report on the characterization of rice osa-miR827 and its two target genes, OsSPX-MFS1 and OsSPX-MFS2, which encode SPX-MFS proteins predicted to be implicated in phosphate (Pi) sensing or transport. We first show by Northern blot analysis that osa-miR827 is strongly induced by Pi starvation in both shoots and roots. Hybridization of osa-miR827 in situ confirms its strong induction by Pi starvation, with signals concentrated in mesophyll, epidermis and ground tissues of roots. In parallel, we analyzed the responses of the two OsSPX-MFS1 and OsSPX-MFS2 gene targets to Pi starvation. OsSPX-MFS1 mRNA is mainly expressed in shoots under sufficient Pi supply while its expression is reduced on Pi starvation, revealing a direct relationship between induction of osa-miR827 and down-regulation of OsSPX-MFS1. In contrast, OsSPX-MFS2 responds in a diametrically opposed manner to Pi starvation. The accumulation of OsSPX-MFS2 mRNA is dramatically enhanced under Pi starvation, suggesting the involvement of complex regulation of osa-miR827 and its two target genes. We further produced transgenic rice lines overexpressing osa-miR827 and T-DNA knockout mutant lines in which the expression of osa-miR827 is abolished. Compared with wild-type controls, both target mRNAs exhibit similar changes, their expression being reduced and increased in overexpressing and knockout lines, respectively. This suggests that OsSPX-MFS1 and OsSPX-MFS2 are both negatively regulated by osa-miR827 abundance although they respond differently to external Pi conditions. We propose that this is a complex mechanism comprising fine tuning of spatial or temporal regulation of both targets by osa-miR827.
引用
收藏
页码:2119 / 2131
页数:13
相关论文
共 58 条
[1]   ProtTest: selection of best-fit models of protein evolution [J].
Abascal, F ;
Zardoya, R ;
Posada, D .
BIOINFORMATICS, 2005, 21 (09) :2104-2105
[2]   Modulation of floral development by a gibberellin-regulated microRNA [J].
Achard, P ;
Herr, A ;
Baulcombe, DC ;
Harberd, NP .
DEVELOPMENT, 2004, 131 (14) :3357-3365
[3]   Transcriptional regulation of phosphate-responsive genes in low-affinity phosphate-transporter-defective mutants in Saccharomyces cerevisiae [J].
Auesukaree, C ;
Homma, T ;
Kaneko, Y ;
Harashima, S .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2003, 306 (04) :843-850
[4]   pho2, a phosphate overaccumulator, is caused by a nonsense mutation in a MicroRNA399 target gene [J].
Aung, Kyaw ;
Lin, Shu-I ;
Wu, Chia-Chune ;
Huang, Yu-Ting ;
Su, Chun-Lin ;
Chiou, Tzyy-Jen .
PLANT PHYSIOLOGY, 2006, 141 (03) :1000-1011
[5]   PHO2, microRNA399, and PHR1 define a phosphate-signaling pathway in plants [J].
Bari, Rajendra ;
Pant, Bikram Datt ;
Stitt, Mark ;
Scheible, Wolf-Ruediger .
PLANT PHYSIOLOGY, 2006, 141 (03) :988-999
[6]   Widespread translational inhibition by plant miRNAs and siRNAs [J].
Brodersen, Peter ;
Sakvarelidze-Achard, Lali ;
Bruun-Rasmussen, Marianne ;
Dunoyer, Patrice ;
Yamamoto, Yoshiharu Y. ;
Sieburth, Leslie ;
Voinnet, Olivier .
SCIENCE, 2008, 320 (5880) :1185-1190
[7]   Regulation of phosphate homeostasis by microRNA in Arabidopsis [J].
Chiou, TJ ;
Aung, K ;
Lin, SI ;
Wu, CC ;
Chiang, SF ;
Su, CL .
PLANT CELL, 2006, 18 (02) :412-421
[8]   Ubiquitin promoter-based vectors for high-level expression of selectable and/or screenable marker genes in monocotyledonous plants [J].
Christensen, AH ;
Quail, PH .
TRANSGENIC RESEARCH, 1996, 5 (03) :213-218
[9]   Characterization of a sub-family of Arabidopsis genes with the SPX domain reveals their diverse functions in plant tolerance to phosphorus starvation [J].
Duan, Ke ;
Yi, Keke ;
Dang, Lei ;
Huang, Hongjie ;
Wu, Wei ;
Wu, Ping .
PLANT JOURNAL, 2008, 54 (06) :965-975
[10]   Mapping the Arabidopsis organelle proteome [J].
Dunkley, TPJ ;
Hester, S ;
Shadforth, IP ;
Runions, J ;
Weimar, T ;
Hanton, SL ;
Griffin, JL ;
Bessant, C ;
Brandizzi, F ;
Hawes, C ;
Watson, RB ;
Dupree, P ;
Lilley, KS .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2006, 103 (17) :6518-6523