Comparison of urinary monitoring, faecal monitoring and erythrocyte analysis of stable isotope labels to determine magnesium absorption in human subjects

被引:23
作者
Bohn, T
Walczyk, T
Davidsson, L
Pritzkow, W
Klingbeil, P
Vogl, J
Hurrell, RF
机构
[1] Swiss Fed Inst & Technol ETH, Inst Food Sci & Nutr, Human Nutr Lab, CH-8803 Ruschlikon, Switzerland
[2] German Fed Inst Mat Res & Testing BAM, D-12205 Berlin, Germany
关键词
magnesium absorption; stable isotopes; faecal monitoring; urinary monitoring; erythrocytes;
D O I
10.1079/BJN20031023
中图分类号
R15 [营养卫生、食品卫生]; TS201 [基础科学];
学科分类号
100403 ;
摘要
We have evaluated urinary monitoring and erythrocyte analysis to determine Mg absorption in human subjects as alternatives to the conventional technique of faecal monitoring by stable-isotope techniques. Ten healthy adults received 2.2 mmol Mg-25 in water, together with wheat bread, followed 15 min later by intravenous injection of 0.6 mmol Mg-26 (day 1). Brilliant blue and Yb (given on day 0 and day 1 respectively) served as qualitative and quantitative faecal markers. Urine was collected for 6 d after test meal intake. Complete collections of faeces were made until excretion of the second brilliant blue marker (given on day 7). Mg isotope ratios were determined by thermal ionisation-MS in urine and faeces and by inductively coupled plasma-MS in erythrocytes. Absorption was determined based on: (1) 6 d urine pools; (2) 24 h urine pools (collected 22-46 h after test meal intake); (3) erythrocytes from a blood sample drawn on day 14; (4) complete 6 d faecal pools; (5) faecal pools based on the first three consecutive stools after excretion of the first brilliant blue marker. Differences in mean Mg absorption (42 44 %) were statistically insignificant between techniques, except when based on 6 d urine pools for which the value was significantly lower (33 (SD 7) %, P=0.0003, ANOVA). The results indicate that Mg absorption can be determined from 24 h urine pools or erythrocytes obtained 14 d after test meal intake, an alternative method to the more time-consuming and labour-intense faecal monitoring. The choice of technique depends on practical and financial considerations.
引用
收藏
页码:113 / 120
页数:8
相关论文
共 25 条
[1]  
ABRAHAM G E, 1991, Journal of Nutritional Medicine, V2, P165, DOI 10.3109/13590849109084112
[2]   Methodologies for using stable isotopes to assess magnesium absorption and secretion in children [J].
Abrams, SA ;
Wen, JP .
JOURNAL OF THE AMERICAN COLLEGE OF NUTRITION, 1999, 18 (01) :30-35
[3]   RADIONUCLIDE TECHNIQUE FOR STUDIES OF ZINC-ABSORPTION IN MAN [J].
ARVIDSSON, B ;
CEDERBLAD, A ;
BJORNRASMUSSEN, E ;
SANDSTROM, B .
INTERNATIONAL JOURNAL OF NUCLEAR MEDICINE & BIOLOGY, 1978, 5 (2-3) :104-109
[4]   MG28 KINETICS IN MAN [J].
AVIOLI, LV ;
BERMAN, M .
JOURNAL OF APPLIED PHYSIOLOGY, 1966, 21 (06) :1688-&
[5]   ABSOLUTE ISOTOPIC ABUNDANCE RATIOS AND ATOMIC WEIGHT OF MAGNESIUM [J].
CATANZAR.EJ ;
MURPHY, TJ ;
GARNER, EL ;
SHIELDS, WR .
JOURNAL OF RESEARCH OF THE NATIONAL BUREAU OF STANDARDS SECTION A-PHYSICS AND CHEMISTRY, 1966, A 70 (06) :453-+
[6]   FOOD IRON-ABSORPTION MEASURED BY AN EXTRINSIC TAG [J].
COOK, JD ;
FINCH, CA ;
WALKER, R ;
MARTINEZ.C ;
LAYRISSE, M ;
MONSEN, E .
JOURNAL OF CLINICAL INVESTIGATION, 1972, 51 (04) :805-+
[7]   Study of magnesium bioavailability using stable isotopes and the inductively-coupled plasma mass spectrometry technique in the rat: Single and double labelling approaches [J].
Coudray, C ;
Pepin, D ;
Tressol, JC ;
Bellanger, J ;
Rayssiguier, Y .
BRITISH JOURNAL OF NUTRITION, 1997, 77 (06) :957-970
[8]  
DEGRAZIA JA, 1965, J LAB CLIN MED, V66, P822
[9]  
Durlach J., 1988, MAGNESIUM CLIN PRACT
[10]   MAGNESIUM - THE 5TH BUT FORGOTTEN ELECTROLYTE [J].
ELIN, RJ .
AMERICAN JOURNAL OF CLINICAL PATHOLOGY, 1994, 102 (05) :616-622