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The nature of the photosystem II reaction centre in the chlorophyll d-containing prokaryote, Acaryochloris marina
被引:60
作者:
Chen, M
Telfer, A
[1
]
Lin, S
Pascal, A
Larkum, AWD
Barber, J
Blankenship, RE
机构:
[1] Univ Sydney, Dept Biol Sci, Sydney, NSW 2006, Australia
[2] Univ London Imperial Coll Sci Technol & Med, Div Mol Biosci, London SW7 2AZ, England
[3] Arizona State Univ, Dept Chem & Biochem, Ctr Study Early Events Photosynth, Tempe, AZ 85287 USA
[4] CEA Saclay, Serv Biophys Fonct Membranaires, DBJC CEA, F-91191 Gif Sur Yvette, France
[5] CEA Saclay, CNRS, URA 2096, F-91191 Gif Sur Yvette, France
关键词:
D O I:
10.1039/b507057k
中图分类号:
Q5 [生物化学];
Q7 [分子生物学];
学科分类号:
071010 ;
081704 ;
摘要:
Pigment-protein complexes enriched in photosystein II (PS II) have been isolated from the chlorophyll (Chl) d containing cyanobacterium, Acaryochloris marina. A small PS II-enriched particle, we call 'crude reaction centre', contained 20 Chl d, 0.5 Chl a and 1 redox active cytochrome b-559 per 2 pheophytin a, plus the D1 and D2 proteins. A larger PS II-enriched particle, we call 'core', additionally bound the antenna complexes, CP47 and CP43, and had a higher chlorophyll per pheophytin ratio. Pheophytin a could be photoreduced in the presence of a strong reductant, indicating that it is the primary electron acceptor in photosystem II of A. marina. A substoichiometric amount of Chl a (less than one chlorophyll a per 2 pheophytin a) strongly suggests that Chl a does not have an essential role in the photochemistry of PS II in this organism. We conclude that PS II, in A. marina, utilizes Chl d and not Chl a as primary electron donor and that the primary electron acceptor is one of two molecules of pheophytin a.
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页码:1060 / 1064
页数:5
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