Two-dimensional electrophoresis of proteins in an immobilized pH 4-12 gradient

被引：78

作者：

Görg, A ^{[1
]}

Boguth, G ^{[1
]}

Obermaier, C ^{[1
]}

Weiss, W ^{[1
]}

机构：

[1] Tech Univ Munchen, Lehrstuhl Allgemeine Lebensmitteltechnol, Dept Food Technol, D-85350 Freising, Germany

来源：

ELECTROPHORESIS | 1998年 / 19卷 / 8-9期

基金：

美国国家科学基金会;

关键词：

alkaline proteins; wide immobilized pH gradient; mouse liver; ribosomal proteins; two-dimensional polyacrylamide gel electrophoresis;

D O I：

10.1002/elps.1150190850

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

For checking theoretical two-dimensional (2-D) maps derived from sequenced genomes, indicating that nonnegligible amounts of proteins up to pH 12 are to be expected, a wide-range immobilized pH 4-12 gradient was generated. Depending on the extraction method of sample preparation, proteins with pls up to pH 12 are detected in a single gel. Highly reproducible protein patterns focused to the steady state with round-shaped spots up to pH 12 are obtained with the standard protocol originally described in 1988 (Gorg et al., Electrophoresis 1988, 9, 531-546).

引用

页码：1516 / 1519

页数：4

共 18 条

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