Protein-repellent nanocomposite with rechargeable calcium and phosphate for long-term ion release

被引:25
作者
Al-Dulaijan, Yousif A. [1 ,2 ]
Weir, Michael D. [1 ]
Melo, Mary Anne S. [1 ]
Sun, Jirun [3 ]
Oates, Thomas W. [1 ]
Zhang, Ke [1 ,4 ]
Xu, Hockin H. K. [1 ,5 ,6 ]
机构
[1] Univ Maryland, Sch Dent, Dept Adv Oral Sci & Therapeut, Baltimore, MD 21201 USA
[2] Imam Abdulrahman Bin Faisal Univ, Coll Dent, Dept Substitut Dent Sci, Dammam, Saudi Arabia
[3] NIST, Amer Dent Assoc Fdn, Volpe Res Ctr, Gaithersburg, MD 20899 USA
[4] Capital Med Univ, Sch Stomatol, Dept Orthodont, Beijing, Peoples R China
[5] Univ Maryland, Sch Med, Ctr Stem Cell Biol & Regenerat Med, Baltimore, MD 21201 USA
[6] Univ Maryland, Sch Med, Marlene & Stewart Greenebaum Canc Ctr, Baltimore, MD 21201 USA
基金
中国国家自然科学基金;
关键词
Dental composite; Calcium phosphate nanoparticles; Long-term ion recharge; Protein-repellent; Oral biofilms; Remineralization; ANTIBACTERIAL DENTAL COMPOSITE; RESTORATIVE MATERIALS; MECHANICAL-PROPERTIES; RESIN-COMPOSITES; SURFACE; POLYMER; CARIES; NANOPARTICLES; ADHESIVE; REMINERALIZATION;
D O I
10.1016/j.dental.2018.09.005
中图分类号
R78 [口腔科学];
学科分类号
100302 [口腔临床医学];
摘要
Objective. There has been no report on the effect of incorporating protein repellent 2-methacryloyloxyethyl phosphorylcholine (MPC) into a composite containing nanoparticles of amorphous calcium phosphate (NACP) on calcium (Ca) and phosphate (P) ion rechargeability. The objectives of this study were to develop a Ca and P ion-rechargeable and protein-repellent composite for the first time, and investigate the effects of MPC and NACP on mechanical properties, protein-repellency, anti-biofilm effects, and Ca and P ion recharge and re-release. Methods. NACP were synthesized using a spray-drying technique. The resin contained ethoxylated bisphenol A dimethacrylate (EBPADMA) and pyromellitic glycerol dimethacrylate (PMGDM). Three NACP composites were made with 0 (control), 1.5%, and 3% of MPC. NACP (20%) and glass particles (50%) were also added into the resin. Protein adsorption was measured using a micro-bicinchoninic acid (BCA) method. A human saliva microcosm biofilm model was used to determine biofilm metabolic activity, lactic acid, and colony-forming units (CFU). Ca and P ion recharge and re-release were measured using a spectrophotometric method. Results. Flexural strengths and moduli of CaP-rechargeable composites matched those of a commercial composite without CaP rechargeability (p > 0.1). Adding 1.5% and 3% MPC reduced protein adsorption to 1/3 and 1/5, respectively, that of commercial composite (p <0.05). Adding 3% MPC suppressed biofilm metabolic activity and lactic acid production, and reduced biofilm CFU by nearly 2 logs. All three NACP composites had excellent ion rechargeability and higher levels of ion re-releases. One recharge yielded continuous ion release for 21 days. The release was maintained at the same level with increasing number of recharge cycles, indicating long-term ion release. Incorporation of MPC did not compromise the CaP ion rechargeability. Significance. Incorporating 3% MPC into NACP nanocomposite greatly reduced protein adsorption, biofilm growth and lactic acid, decreasing biofilm CFU by nearly 2 logs, without compromising Ca and P recharge. This protein-repellent NACP-MPC rechargeable composite with long-term remineralization is promising for tooth restorations to inhibit secondary caries. (C) 2018 The Academy of Dental Materials. Published by Elsevier Inc. All rights reserved.
引用
收藏
页码:1735 / 1747
页数:13
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