Chromatography of plasmid DNA

被引：147

作者：

Diogo, MM

Queiroz, JA

Prazeres, DMF

机构：

[1] Inst Super Tecn, Ctr Engn Biol & Quim, P-1049001 Lisbon, Portugal

[2] Univ Beira Interior, Ctr Invest Ciencias Saude, P-6200001 Covilha, Portugal

来源：

JOURNAL OF CHROMATOGRAPHY A | 2005年 / 1069卷 / 01期

关键词：

plasmid DNA; gene therapy; purification; chromatography; RNA; genomic DNA; endotoxins;

D O I：

10.1016/j.chroma.2004.09.050

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Liquid chromatography plays a central role in process-scale manufacturing of therapeutic plasmid DNA (pDNA) for gene therapy and DNA vaccination. Apart from its use as a preparative purification step, it is also very useful as an analytical tool to monitor and control pDNA quality during processing and in final formulations. This paper gives an overview of the use of pDNA chromatography. The specificity of pDNA purification and the consequent limitations to the performance of chromatography are described. Strategies currently used to overcome those limitations, as well as other possible solutions are presented. Applications of the different types of chromatography to the purification of therapeutic pDNA are reviewed, and the main advantages and disadvantages behind each technique highlighted. (c) 2004 Elsevier B.V. All rights reserved.

引用

页码：3 / 22

页数：20

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