Glycosylation beyond the Asn78-linked GlcNAc residue has a significant enhancing effect on the stability of the alpha subunit of human chorionic gonadotropin

被引:32
作者
vanZuylen, CWEM [1 ]
Kamerling, JP [1 ]
Vliegenthart, JFG [1 ]
机构
[1] UNIV UTRECHT,BIJVOET CTR,DEPT BIOORGAN CHEM,NL-3508 TB UTRECHT,NETHERLANDS
关键词
D O I
10.1006/bbrc.1997.6241
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The effects of glycosylation beyond the Asn-linked GlcNAc residues on the stability of the alpha subunit of human chorionic gonadotropin are investigated, using enzymatic deglycosylation and NMR spectroscopy. Comparison of thermal denaturation profiles of both the intact alpha subunit and the alpha subunit carrying only GlcNAc monomers at both Asn52 and Asn78 established a small but significant decrease in thermal stability of the deglycosylated form. Since there is no secondary structure around Asn52 in the free subunit these results demonstrate that glycosylation beyond the Asn78-linked GlcNAc residue enhances the thermal stability of the alpha subunit of hCG. This feature has implications for understanding the effect of glycosylation on protein stabilization in general. (C) 1997 Academic Press.
引用
收藏
页码:117 / 120
页数:4
相关论文
共 22 条
[1]   MLEV-17-BASED TWO-DIMENSIONAL HOMONUCLEAR MAGNETIZATION TRANSFER SPECTROSCOPY [J].
BAX, A ;
DAVIS, DG .
JOURNAL OF MAGNETIC RESONANCE, 1985, 65 (02) :355-360
[2]   COHERENCE TRANSFER BY ISOTROPIC MIXING - APPLICATION TO PROTON CORRELATION SPECTROSCOPY [J].
BRAUNSCHWEILER, L ;
ERNST, RR .
JOURNAL OF MAGNETIC RESONANCE, 1983, 53 (03) :521-528
[3]   CD2: An exception to the immunoglobulin superfamily concept? [J].
Davis, SJ ;
vanderMerwe, PA .
SCIENCE, 1996, 273 (5279) :1241-1242
[4]  
DeBeer T, 1996, EUR J BIOCHEM, V241, P229
[5]  
DWEK RA, 1995, BIOCHEM SOC T, V23, P1
[6]   CLEAN TOCSY FOR H-1 SPIN SYSTEM-IDENTIFICATION IN MACROMOLECULES [J].
GRIESINGER, C ;
OTTING, G ;
WUTHRICH, K ;
ERNST, RR .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1988, 110 (23) :7870-7872
[7]   THE ASN-LINKED CARBOHYDRATE CHAINS OF HUMAN TAMM-HORSFALL GLYCOPROTEIN OF ONE MALE - NOVEL SULFATED AND NOVEL N-ACETYLGALACTOSAMINE-CONTAINING N-LINKED CARBOHYDRATE CHAINS [J].
HARD, K ;
VANZADELHOFF, G ;
MOONEN, P ;
KAMERLING, JP ;
VLIEGENTHART, JFG .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1992, 209 (03) :895-915
[8]   CYSTINE KNOTS [J].
ISAACS, NW .
CURRENT OPINION IN STRUCTURAL BIOLOGY, 1995, 5 (03) :391-395
[9]   EFFECTS OF GLYCOSYLATION ON PROTEIN-STRUCTURE AND DYNAMICS IN RIBONUCLEASE-B AND SOME OF ITS INDIVIDUAL GLYCOFORMS [J].
JOAO, HC ;
DWEK, RA .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1993, 218 (01) :239-244
[10]   CRYSTAL-STRUCTURE OF HUMAN CHORIONIC-GONADOTROPIN [J].
LAPTHORN, AJ ;
HARRIS, DC ;
LITTLEJOHN, A ;
LUSTBADER, JW ;
CANFIELD, RE ;
MACHIN, KJ ;
MORGAN, FJ ;
ISAACS, NW .
NATURE, 1994, 369 (6480) :455-461