Sequence differences in the diagnostic target region of the oocyst wall protein gene of Cryptosporidium parasites

被引:103
作者
Xiao, LH
Limor, J
Morgan, UM
Sulaiman, IM
Thompson, RAC
Lal, AA
机构
[1] US Dept HHS, Publ Hlth Serv, Ctr Dis Control & Prevent, Natl Ctr Infect Dis,Div Parasit Dis, Atlanta, GA 30341 USA
[2] Murdoch Univ, State Agr Biotechnol Ctr, Murdoch, WA 6150, Australia
关键词
D O I
10.1128/AEM.66.12.5499-5502.2000
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Nucleotide sequences of the Cryptosporidium oocyst wall protein (COWP) gene were obtained from various Cryptosporidium spp, (C. wrairi, C. felis, C. meleagridis, C, baileyi, C, andersoni, C. muris, and C,serpentis) and C, parvum genotypes (human, bovine, monkey, marsupial, ferret, mouse, pig, and dog). Significant diversity was observed among species and genotypes in the primer and target regions of a popular diagnostic PCR, These results provide useful information for COWP-based molecular differentiation of Cryptosporidium spp, and genotypes.
引用
收藏
页码:5499 / 5502
页数:4
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