Anastellin, an FN3 fragment with fibronectin polymerization activity, resembles amyloid fibril precursors

被引:63
作者
Briknarová, K
Åkerman, ME
Hoyt, DW
Ruoslahti, E
Ely, KR
机构
[1] Burnham Inst, La Jolla, CA 92037 USA
[2] Univ Calif San Diego, Dept Bioengn, La Jolla, CA 92093 USA
[3] EMSL, Pacific NW Natl Lab, Richland, WA 99352 USA
关键词
amyloid fibril; anastellin; extracellular matrix; fibronectin type 3 (FN3) domain; NMR;
D O I
10.1016/S0022-2836(03)00890-8
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Anastellin is a carboxy-terminal fragment of the first FN3 domain from human fibronectin. It is capable of polymerizing fibronectin in vitro, and it displays anti-tumor, anti-metastatic and anti-angiogenic properties in vivo. We have determined the structure of anastellin using nuclear magnetic resonance spectroscopy and identified residues critical for its activity. Anastellin exhibits dynamic fluctuations and conformational exchange in solution. Its overall topology is very similar to the corresponding region of full-length FN3 domains. However, its hydrophobic core becomes solvent-accessible and some of its beta-strands lose their protection against hydrogen bonding to beta-strands from other molecules. These features seem to be relevant for the fibronectin polymerization activity of anastellin and resemble the characteristics of amyloid fibril precursors. We suggest that this analogy is not random and may reflect similarities between fibronectin and amyloid fibril formation. (C) 2003 Elsevier Ltd. All rights reserved.
引用
收藏
页码:205 / 215
页数:11
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