Organization and structure of NADH-dependent glutamate synthase gene from rice plants

被引：39

作者：

Goto, S ^{[1
]}

Akagawa, T ^{[1
]}

Kojima, S ^{[1
]}

Hayakawa, T ^{[1
]}

Yamaya, T ^{[1
]}

机构：

[1] Tohoku Univ, Grad Sch Agr Sci, Dept Life Sci, Aoba Ku, Sendai 9818555, Japan

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY | 1998年 / 1387卷 / 1-2期

基金：

日本学术振兴会;

关键词：

cDNA; gene structure; NADH glutamate synthase; (rice; Oryza sativa);

D O I：

10.1016/S0167-4838(98)00142-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Genomic clones for NADH-dependent glutamate synthase (NADH-GOGAT; EC 1.4.1.14) were obtained from a genomic library of rice (Oryza sativa L. cv. Sasanishki). A genomic clone (lambda OS42, 14 kb) covered an entire structural gene and a 3.7 kb 5'-upstream region from the first methionine. Another clone (lambda OS23, 14 kb) contained a 2.8 kb 3'-downstream region from the stop codon. A 7047 bp long clone (lambda OSR51) consisting of full length cDNA for NADH-GOGAT was isolated from a cDNA library prepared using mRNA from roots of rice seedlings treated with 1 mM NH4Cl for 12 h. The presumed transcribed region (11.7 kb) consisted of 23 exons separated by 22 introns. Rice NADH-GOGAT is synthesized as a 2166 amino acid protein with a molecular mass of 236.7 kDa that includes a 99 amino acid presequence. DNA gel blot analysis suggested that NADH-GOGAT occurred as a single gene in rice. Primer extension experiments map the transcription start of NADH-GOGAT to identical positions. The 3.7 kb 5'-upstream region was able to transiently express a reporter gene in cultured rice cells. Putative motifs related to the regulation of NADH-GOGAT gene expression were looked for within the 5'-upstream region by database. (C) 1998 Elsevier Science B.V. All rights reserved.

引用

页码：298 / 308

页数：11

共 41 条

[31] DNA SEQUENCING WITH CHAIN-TERMINATING INHIBITORS [J].

SANGER, F ;

NICKLEN, S ;

COULSON, AR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1977, 74 (12) :5463-5467

[32] COMPARTMENTATION OF NITROGEN ASSIMILATION IN HIGHER-PLANTS [J].

SECHLEY, KA ;

YAMAYA, T ;

OAKS, A .

INTERNATIONAL REVIEW OF CYTOLOGY-A SURVEY OF CELL BIOLOGY, 1992, 134 :85-163

[33] THE ATHB-1 AND ATHB-2 HD-ZIP DOMAINS HOMODIMERIZE FORMING COMPLEXES OF DIFFERENT DNA-BINDING SPECIFICITIES [J].

SESSA, G ;

MORELLI, G ;

RUBERTI, I .

EMBO JOURNAL, 1993, 12 (09) :3507-3517

[34] NUCLEOTIDE-SEQUENCE OF THE LIPOAMIDE DEHYDROGENASE GENE OF ESCHERICHIA-COLI-K12 [J].

STEPHENS, PE ;

LEWIS, HM ;

DARLISON, MG ;

GUEST, JR .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1983, 135 (03) :519-527

[35] Structure and regulation of ferredoxin-dependent glutamase synthase from Arabidopsis thaliana - Cloning of cDNA, expression in different tissues of wild-type and gltS mutant strains, and light induction [J].

Suzuki, A ;

Rothstein, S .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1997, 243 (03) :708-718

[36]

TSO JY, 1982, J BIOL CHEM, V257, P3525

[37] ALFALFA NADH-DEPENDENT GLUTAMATE SYNTHASE - STRUCTURE OF THE GENE AND IMPORTANCE IN SYMBIOTIC N-2 FIXATION [J].

VANCE, CP ;

MILLER, SS ;

GREGERSON, RG ;

SAMAC, DA ;

ROBINSON, DL ;

GANTT, JS .

PLANT JOURNAL, 1995, 8 (03) :345-358

[38] Expression of NADH-dependent glutamate synthase in response to the supply of nitrogen in rice cells in suspension culture [J].

Watanabe, S ;

Sakai, T ;

Goto, S ;

Yaginuma, T ;

Hayakawa, T ;

Yamaya, T .

PLANT AND CELL PHYSIOLOGY, 1996, 37 (07) :1034-1037

[39]

YAMAYA T, 1995, PLANT CELL PHYSIOL, V36, P1197

[40] TISSUE DISTRIBUTION OF GLUTAMATE SYNTHASE AND GLUTAMINE-SYNTHETASE IN RICE LEAVES - OCCURRENCE OF NADH-DEPENDENT GLUTAMATE SYNTHASE PROTEIN AND ACTIVITY IN THE UNEXPANDED, NONGREEN LEAF BLADES [J].

YAMAYA, T ;

HAYAKAWA, T ;

TANASAWA, K ;

KAMACHI, K ;

MAE, T ;

OJIMA, K .

PLANT PHYSIOLOGY, 1992, 100 (03) :1427-1432

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