Organization and structure of NADH-dependent glutamate synthase gene from rice plants

被引：39

作者：

Goto, S ^{[1
]}

Akagawa, T ^{[1
]}

Kojima, S ^{[1
]}

Hayakawa, T ^{[1
]}

Yamaya, T ^{[1
]}

机构：

[1] Tohoku Univ, Grad Sch Agr Sci, Dept Life Sci, Aoba Ku, Sendai 9818555, Japan

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY | 1998年 / 1387卷 / 1-2期

基金：

日本学术振兴会;

关键词：

cDNA; gene structure; NADH glutamate synthase; (rice; Oryza sativa);

D O I：

10.1016/S0167-4838(98)00142-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Genomic clones for NADH-dependent glutamate synthase (NADH-GOGAT; EC 1.4.1.14) were obtained from a genomic library of rice (Oryza sativa L. cv. Sasanishki). A genomic clone (lambda OS42, 14 kb) covered an entire structural gene and a 3.7 kb 5'-upstream region from the first methionine. Another clone (lambda OS23, 14 kb) contained a 2.8 kb 3'-downstream region from the stop codon. A 7047 bp long clone (lambda OSR51) consisting of full length cDNA for NADH-GOGAT was isolated from a cDNA library prepared using mRNA from roots of rice seedlings treated with 1 mM NH4Cl for 12 h. The presumed transcribed region (11.7 kb) consisted of 23 exons separated by 22 introns. Rice NADH-GOGAT is synthesized as a 2166 amino acid protein with a molecular mass of 236.7 kDa that includes a 99 amino acid presequence. DNA gel blot analysis suggested that NADH-GOGAT occurred as a single gene in rice. Primer extension experiments map the transcription start of NADH-GOGAT to identical positions. The 3.7 kb 5'-upstream region was able to transiently express a reporter gene in cultured rice cells. Putative motifs related to the regulation of NADH-GOGAT gene expression were looked for within the 5'-upstream region by database. (C) 1998 Elsevier Science B.V. All rights reserved.

引用

页码：298 / 308

页数：11