Quorum sensing signal molecules (acylated homoserine lactones) in Gram-negative fish pathogenic bacteria

被引:95
作者
Bruhn, JB
Dalsgaard, I
Nielsen, KF
Buchholtz, C
Larsen, JL
Gram, L
机构
[1] CO Tech Univ Denmark, Dept Seafood Res, Danish Inst Fisheries, DK-2800 Lyngby, Denmark
[2] Danish Inst Fisheries, Fish Dis Lab, DK-1870 Frederiksberg, Denmark
[3] Tech Univ Denmark, Ctr Microbial Biotechnol, DK-2800 Lyngby, Denmark
[4] Royal Vet & Agr Univ, Dept Vet Pathobiol, DK-1870 Frederiksberg, Denmark
关键词
quorum sensing; signal molecules; acylated homoserine lactones; AHL; fish pathogenic bacteria;
D O I
10.3354/dao065043
中图分类号
S9 [水产、渔业];
学科分类号
0908 ;
摘要
The aim of the present study was to investigate the production of quorum sensing signals (specifically acylated homoserine lactones, AHLs) among a selection of strains of Gram-negative fish bacterial pathogens. These signals are involved in the regulation of virulence factors in some human and plant-pathogenic bacteria. A total of 59 strains, representing 9 different fish pathogenic species, were tested against 2 AHL monitor bacteria (Agrobacterium tumefaciens NT1 [pZLR4] and Chromobacterium violaceum CV026) in a well diffusion assay and by thin-layer chromatography (TLC). Representative samples were further characterized by high performance liquid chromatography-high resolution mass spectrometry (HPLC-HR-MS). AHLs were produced by all strains of Aeromonas salmonicida, Aeromonas hydrophila, Yersinia ruckeri, Vibrio salmonicida, and Vibrio vulnificus. Some strains of atypical Aeromonas salmonicida and Vibrio splendidus were also positive. Aeromonas species produced N-butanoyl homoserine lactone (BHL) and N-hexanoyl homoserine lactone (HHL) and 1 additional product, whereas N-3-oxo-hexanoyl homoserine lactone (OHHL) and HHL were detected in Vibrio salmonicida. N-3-oxo-octanoyl homoserine lactone (OOHL) and N-3-octanoyl homoserine lactone (OHL) were detected in Y. ruckeii. AHLs were not detected from strains of Photobacterium damselae, Flavobacterium psychrophilum or Moritella viscosa. AHLs were extracted from fish infected with Y. ruckeri but not from fish infected with A. salmonicida. In conclusion, the production of quorum sensing signals, AHLs, is common among the strains that we examined. If the AHL molecules regulate the expression of the virulence phenotype in these bacteria, as shown to occur in some bacterial pathogens, novel disease control measures may be developed by blocking AHL-mediated communication and suppressing virulence.
引用
收藏
页码:43 / 52
页数:10
相关论文
共 43 条
[1]   Salmonella typhimurium encodes an SdiA homolog, a putative quorum sensor of the LuxR family, that regulates genes on the virulence plasmid [J].
Ahmer, BMM ;
van Reeuwijk, J ;
Timmers, CD ;
Valentine, PJ ;
Heffron, F .
JOURNAL OF BACTERIOLOGY, 1998, 180 (05) :1185-1193
[2]   Production of acylated homoserine lactones by different serotypes of Vibrio anguillarum both in culture and during infection of rainbow trout [J].
Buch, C ;
Sigh, J ;
Nielsen, J ;
Larsen, JL ;
Gram, L .
SYSTEMATIC AND APPLIED MICROBIOLOGY, 2003, 26 (03) :338-349
[3]  
CAO JG, 1989, J BIOL CHEM, V264, P21670
[4]   Production of acyl-homoserine lactone quorum-sensing signals by gram-negative plant-associated bacteria [J].
Cha, C ;
Gao, P ;
Chen, YC ;
Shaw, PD ;
Farrand, SK .
MOLECULAR PLANT-MICROBE INTERACTIONS, 1998, 11 (11) :1119-1129
[5]   Structural identification of a bacterial quorum-sensing signal containing boron [J].
Chen, X ;
Schauder, S ;
Potier, N ;
Van Dorsselaer, A ;
Pelczer, I ;
Bassler, BL ;
Hughson, FM .
NATURE, 2002, 415 (6871) :545-549
[6]   Quorum-sensing-directed protein expression in Serratia proteamaculans B5a [J].
Christensen, AB ;
Riedel, K ;
Eberl, L ;
Flodgaard, LR ;
Molin, S ;
Gram, L ;
Givskov, M .
MICROBIOLOGY-SGM, 2003, 149 :471-483
[7]   VanT, a homologue of Vibrio harveyi LuxR, regulates serine, metalloprotease, pigment, and biofilm production in Vibrio anguillarum [J].
Croxatto, A ;
Chalker, VJ ;
Lauritz, J ;
Jass, J ;
Hardman, A ;
Williams, P ;
Cámara, M ;
Milton, DL .
JOURNAL OF BACTERIOLOGY, 2002, 184 (06) :1617-1629
[8]   Bacterial quorum sensing in pathogenic relationships [J].
de Kievit, TR ;
Iglewski, BH .
INFECTION AND IMMUNITY, 2000, 68 (09) :4839-4849
[9]   Plant growth-promoting Pseudomonas putida WCS358 produces and secretes four cyclic dipeptides:: Cross-talk with quorum sensing bacterial sensors [J].
Degrassi, G ;
Aguilar, C ;
Bosco, M ;
Zahariev, S ;
Pongor, S ;
Venturi, V .
CURRENT MICROBIOLOGY, 2002, 45 (04) :250-254
[10]   EFFECT OF OXYTETRACYCLINE-MEDICATED FEED ON ANTIBIOTIC-RESISTANCE OF GRAM-NEGATIVE BACTERIA IN CATFISH PONDS [J].
DEPAOLA, A ;
PEELER, JT ;
RODRICK, GE .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1995, 61 (06) :2335-2340