Dual inhibitory effects of dimethyl sulfoxide on poly(ADP-ribose) synthetase

被引：18

作者：

Banasik, M

Ueda, K ^{[1
]}

机构：

[1] Kyoto Univ, Inst Chem Res, Lab Mol Clin Chem, Uji, Kyoto 6110011, Japan

[2] Polish Acad Sci, Inst Environm Engn, PL-41819 Zabrze, Poland

来源：

JOURNAL OF ENZYME INHIBITION | 1999年 / 14卷 / 03期

基金：

日本学术振兴会;

关键词：

poly(ADP-ribose) polymerase; ADP-ribosyltransferase; NAD(+); benzamide; 1,5-dihydroxyisoquinoline; 6(5H)-phenanthridinone;

D O I：

10.3109/14756369909030319

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Dimethyl sulfoxide (DMSO), a solvent popularly used for dissolving water-insoluble compounds, is a weak inhibitor of poly(ADP-ribose) synthetase, that is a nuclear enzyme producing (ADP-ribose)(n) from NAD(+). The inhibitory mode and potency depend on the concentration of substrate, NAD(+), as well as the temperature of the reaction; at micromolar concentrations of NAD(+), the inhibition by DMSO is biphasic at 37 degrees C, but is monophasic and apparently competitive with NAD(+) at 25 degrees C. DMSO, on the other hand, diminishes dose-dependently and markedly the inhibitory potency of benzamide and other inhibitors. Other organic solvents, ethanol and methanol, also show a biphasic effect on the synthetase activity at different concentrations.

引用

页码：239 / 250

页数：12

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