Amperometric hydrogen peroxide biosensor with silica sol-gel/chitosan film as immobilization matrix

被引：136

作者：

Miao, Y ^{[1
]}

Tan, SN ^{[1
]}

机构：

[1] Nanyang Technol Univ, Acad Grp Nat Sci, Singapore 637616, Singapore

来源：

ANALYTICA CHIMICA ACTA | 2001年 / 437卷 / 01期

关键词：

silica sol-gel/chitosan composite film; horseradish peroxidase; biosensor; hydrogen peroxide;

D O I：

10.1016/S0003-2670(01)00986-2

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A new type of silica sol-gel/organic hybrid composite material was developed for the fabrication of an amperometric H2O2 biosensor. This composite consists of sol-gel and the natural polymer chitosan. The composite film was used to immobilize horseradish peroxidase on a carbon paste electrode. The fabrication procedure was systematically optimized to improve the biosensor performance. Hexacyanoferrate(II) was present in the solution as an electron donor. The biosensor had a fast response of less than 10 s and linear calibration range of 2.5 x 10(-4) to 3.4 x 10(-3) M, with a detection limit of 3 muM based on S/N = 3. The biosensor retained ca. 85% of its original activity after 30 days of storage in a phosphate buffer at 4 degreesC. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：87 / 93

页数：7

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