Identification of major compounds in rat bile after oral administration of total triterpenoids of Ganoderma lucidum by high-performance liquid chromatography with electrospray ionization tandem mass spectrometry

被引：30

作者：

Guo, Xiao-Yu ^{[1
]}

Han, Jian ^{[1
]}

Ye, Min ^{[1
]}

Ma, Xiao-Chi ^{[2
]}

Shen, Xuan ^{[1
]}

Xue, Bin-Bin ^{[1
]}

Che, Qing-Ming ^{[1
]}

机构：

[1] Peking Univ, Sch Pharmaceut Sci, Dept Nat Med, Beijing 100191, Peoples R China

[2] Dalian Med Univ, Sch Pharm, Dalian 116044, Peoples R China

来源：

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 2012年 / 63卷

基金：

中国国家自然科学基金;

关键词：

Ganoderma lucidum; Triterpenoids; Metabolites; HPLC-DAD-ESI-MSn; LC-ESI-IT-TOF/MS; FR KARST; QUANTITATIVE-DETERMINATION; ACID-F; TERPENOIDS; CONSTITUENTS; SPORES;

D O I：

10.1016/j.jpba.2012.01.030

中图分类号：

O65 [分析化学];

学科分类号：

070302 [分析化学];

摘要：

Triterpenoids are the main bioactive components of Ganoderma lucidum, a famous traditional Chinese medicine. After oral administration of total triterpenoids of G. lucidum (TTGL), the rat bile was analyzed by high-performance liquid chromatography coupled with diode array detection and electrospray ion trap tandem mass spectrometry (HPLC-DAD-ESI-MSn) and liquid chromatography coupled with electrospray ionization hybrid ion trap and time-of-flight mass spectrometry (LC-ESI-IT-TOF/MS). From rat bile and TTGL samples, a total of 31 triterpenoids, including seven new compounds, were identified or tentatively characterized based on their fragmentation behaviors. Among them, 22 triterpenoids were identified from TTGL and 29 triterpenoids were detected from rat bile after oral administration of TTGL. The results indicated that the majority of triterpenoids detected in TTGL extract could be excreted through rat bile. It is the first report on excretion of total triterpenoids of G. lucidum in rat bile. (C) 2012 Elsevier B.V. All rights reserved.

引用

页码：29 / 39

页数：11

共 35 条

[1]

Chen X., 2010, J CHIN PHARM SCI, V19, P209

[2]

Chen Z.B., 2001, MODERN RES GANODERMA, P219

[3]

Fragmentation pathways of oxygenated tetracyclic triterpenoids and their application in the qualitative analysis of Ganoderma lucidum by multistage tandem mass spectrometry [J].