Self-reinforcing activation of a cell-specific transcription factor by proteolysis of an anti-σ factor in B-subtilis

被引：89

作者：

Pan, Q ^{[1
]}

Garsin, DA ^{[1
]}

Losick, R ^{[1
]}

机构：

[1] Harvard Univ, Dept Mol & Cellular Biol, Biol Labs, Cambridge, MA 02138 USA

来源：

MOLECULAR CELL | 2001年 / 8卷 / 04期

关键词：

D O I：

10.1016/S1097-2765(01)00362-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The transcription factor sigma (F), which is activated in a cell-specific manner during sporulation in B. subtilis, is initially held in an inactive complex by the anti-sigma factor SpollAB. The anti-anti-sigma factor SpollAA reacts with SpollAB(.)sigma (F) to induce the release of free sigma (F) and free SpollAB. We now report that free SpollAB is subject to proteolysis and that it is protected from degradation by sigma (F) in the SpollAB(.)sigma (F) complex and by SpollAA in an alternative complex. Proteolysis requires residues located near the extreme C terminus of SpollAB and is dependent upon the ClpCP protease. The reaction of SpollAA with SpollAB(.)sigma (F) and the resulting degradation of newly released SpollAB could set up a self-reinforcing cycle that locks on the activation of sigma (F).

引用

页码：873 / 883

页数：11

共 45 条

[31] SIGMA(F), THE 1ST COMPARTMENT-SPECIFIC TRANSCRIPTION FACTOR OF BACILLUS-SUBTILIS, IS REGULATED BY AN ANTI-SIGMA FACTOR THAT IS ALSO A PROTEIN-KINASE [J].

MIN, KT ;

HILDITCH, CM ;

DIEDERICH, B ;

ERRINGTON, J ;

YUDKIN, MD .

CELL, 1993, 74 (04) :735-742

[32] Modulation of the Escherichia coli sigma(E) (RpoE) heat-shock transcription-factor activity by the RseA, RseB and RseC proteins [J].

Missiakas, D ;

Mayer, MP ;

Lemaire, M ;

Georgopoulos, C ;

Raina, S .

MOLECULAR MICROBIOLOGY, 1997, 24 (02) :355-371

[33] MECB OF BACILLUS-SUBTILIS, A MEMBER OF THE CLPC ATPASE FAMILY, IS A PLEIOTROPIC REGULATOR CONTROLLING COMPETENCE GENE-EXPRESSION AND GROWTH AT HIGH-TEMPERATURE [J].

MSADEK, T ;

KUNST, F ;

RAPOPORT, G .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (13) :5788-5792

[34] ClpP of Bacillus subtilis is required for competence development, motility, degradative enzyme synthesis, growth at high temperature and sporulation [J].

Msadek, T ;

Dartois, V ;

Kunst, F ;

Herbaud, ML ;

Denizot, F ;

Rapoport, G .

MOLECULAR MICROBIOLOGY, 1998, 27 (05) :899-914

[35] SITE OF PHOSPHORYLATION OF SPOIIAA, THE ANTI-ANTI-SIGMA FACTOR FOR SPORULATION-SPECIFIC SIGMA(F) OF BACILLUS-SUBTILIS [J].

NAJAFI, SMA ;

WILLIS, AC ;

YUDKIN, MD .

JOURNAL OF BACTERIOLOGY, 1995, 177 (10) :2912-2913

[36] Sequencing and phylogenetic analysis of the spoIIA operon from diverse Bacillus and Paenibacillus species [J].

Park, SG ;

Yudkin, MD .

GENE, 1997, 194 (01) :25-33

[37] The N- and C-terminal domains of MecA recognize different partners in the competence molecular switch [J].

Persuh, M ;

Turgay, K ;

Mandic-Mulec, I ;

Dubnau, D .

MOLECULAR MICROBIOLOGY, 1999, 33 (04) :886-894

[38] CONTROL OF DEVELOPMENTAL TRANSCRIPTION FACTOR-RHO-F BY SPORULATION REGULATORY PROTEINS SPOIIAA AND SPOIIAB IN BACILLUS-SUBTILIS [J].

SCHMIDT, R ;

MARGOLIS, P ;

DUNCAN, L ;

COPPOLECCHIA, R ;

MORAN, CP ;

LOSICK, R .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (23) :9221-9225

[39] Molecular genetics of sporulation in Bacillus subtilis [J].

Stragier, P ;

Losick, R .

ANNUAL REVIEW OF GENETICS, 1996, 30 :297-341

[40] Biochemical characterization of a molecular switch involving the heat shock protein ClpC, which controls the activity of ComK, the competence transcription factor of Bacillus subtilis [J].

Turgay, K ;

Hamoen, LW ;

Venema, G ;

Dubnau, D .

GENES & DEVELOPMENT, 1997, 11 (01) :119-128

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