Direct conversion of ethane to ethanol by engineered cytochrome P450BM3

被引：118

作者：

Meinhold, P ^{[1
]}

Peters, MW ^{[1
]}

Chen, MMY ^{[1
]}

Takahashi, K ^{[1
]}

Arnold, FH ^{[1
]}

机构：

[1] CALTECH, Div Chem & Chem Engn, Pasadena, CA 91125 USA

来源：

CHEMBIOCHEM | 2005年 / 6卷 / 10期

关键词：

alkanes; cytochromes; directed evolution; enzyme catalysis; hydroxylation;

D O I：

10.1002/cbic.200500261

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Picking on someone smaller. Cytochromes P450 catalyze the hydroxylation of thousands of substrates, including alkanes. No naturally occurring P450, however, is known to oxidize the smallest alkanes, ethane and methane. Here we report the direct and selective oxidation of ethane to ethanol using di-oxygen, catalyzed by a cytochrome P450 BM-3 variant engineered for high activity towards small alkanes (see scheme). Achieving P450-catalyzed oxidation of ethane is a key step in the pathway to P450-catalyzed methane oxidation and opens new opportunities for the bioconversion of natural gas to fuels and chemicals. © 2005 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

页码：1765 / 1768

页数：4

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