RNA LEGO: Magnesium-dependent formation of specific RNA assemblies through kissing interactions

被引:49
作者
Horiya, S
Li, XG
Kawai, G
Saito, R
Katoh, A
Kobayashi, K
Harada, K [1 ]
机构
[1] Tokyo Gakugei Univ, Dept Life Sci, Koganei, Tokyo 1848501, Japan
[2] Chiba Inst Technol, Dept Ind Chem, Narashino, Chiba 2750016, Japan
[3] Seikei Univ, Fac Engn, Dept Appl Chem, Musashino, Tokyo 1808633, Japan
来源
CHEMISTRY & BIOLOGY | 2003年 / 10卷 / 07期
关键词
D O I
10.1016/S1074-5521(03)00146-7
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The high affinity and specificity of nucleic acid base complementarity has been proven to be a powerful method for constructing specific molecular assemblies. On the other hand, recent structural studies of RNA have revealed the wide range of tertiary interactions utilized in RNA folding, which may potentially be used as tools for the design of specific macromolecular assemblies. Here, RNA building blocks containing two hairpin loops, based on the dimerization initiation site (DIS) of HIV RNA, connected by a short linker were used to construct large RNA assemblies through hairpin loop-loop ("kissing") interactions. We show that specific linear and circular assemblies can be constructed in a magnesium-dependent manner using several non-self-complementary loop-loop interactions designed in this study. These results show that the use of RNA tertiary interactions may broaden the reportoire of nucleic acid-based nanostructures.
引用
收藏
页码:645 / 654
页数:10
相关论文
共 41 条
  • [11] DETERMINANTS OF RNA HAIRPIN LOOP-LOOP COMPLEX STABILITY
    GREGORIAN, RS
    CROTHERS, DM
    [J]. JOURNAL OF MOLECULAR BIOLOGY, 1995, 248 (05) : 968 - 984
  • [12] Stitching together RNA tertiary architectures
    Hermann, T
    Patel, DJ
    [J]. JOURNAL OF MOLECULAR BIOLOGY, 1999, 294 (04) : 829 - 849
  • [13] Long-range interaction between the P2.1 and P9.1 peripheral domains of the tetrahymena ribozyme
    Ikawa, Y
    Ohta, H
    Shiraishi, H
    Inoue, T
    [J]. NUCLEIC ACIDS RESEARCH, 1997, 25 (09) : 1761 - 1765
  • [14] Jaeger L, 2000, ANGEW CHEM INT EDIT, V39, P2521, DOI 10.1002/1521-3773(20000717)39:14<2521::AID-ANIE2521>3.0.CO
  • [15] 2-P
  • [16] TectoRNA: modular assembly units for the construction of RNA nano-objects
    Jaeger, L
    Westhof, E
    Leontis, NB
    [J]. NUCLEIC ACIDS RESEARCH, 2001, 29 (02) : 455 - 463
  • [17] Dimerization of HIV-1 genomic RNA of subtypes A and B: RNA loop structure and magnesium binding
    Jossinet, F
    Paillart, JC
    Westhof, E
    Hermann, T
    Skripkin, E
    Lodmell, JS
    Ehresmann, C
    Ehresmann, B
    Marquet, R
    [J]. RNA, 1999, 5 (09) : 1222 - 1234
  • [18] FROM MACROMOLECULES TO BIOLOGICAL ASSEMBLIES (NOBEL LECTURE)
    KLUG, A
    [J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION IN ENGLISH, 1983, 22 (08): : 565 - 582
  • [19] Kissing-loop model of HIV-1 genome dimerization: HTV-1 RNAs can assume alternative dimeric forms, and all sequences upstream or downstream of hairpin 248-271 are dispensable for dimer formation
    Laughrea, M
    Jette, L
    [J]. BIOCHEMISTRY, 1996, 35 (05) : 1589 - 1598
  • [20] New loop-loop tertiary interactions in self-splicing introns of subgroup IC and ID: A complete 3D model of the Tetrahymena thermophila ribozyme
    Lehnert, V
    Jaeger, L
    Michel, F
    Westhof, E
    [J]. CHEMISTRY & BIOLOGY, 1996, 3 (12): : 993 - 1009