Simple and sensitive high-performance liquid chromatographic determination of methamphetamines in human urine as derivatives of 4-(4,5-diphenyl-1H-imidazol-2-yl) benzoyl chloride, a new fluorescence derivatization reagent

被引:29
作者
Al-Dirbashi, O
Qvarnstrom, J
Irgum, K
Nakashima, K
机构
[1] Nagasaki Univ, Sch Pharmaceut Sci, Nagasaki 852, Japan
[2] Umea Univ, Dept Analyt Chem, S-90187 Umea, Sweden
来源
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 1998年 / 712卷 / 1-2期
关键词
human urine; methamphetamine; 4-(4,5-diphenyl-1H-imidazol-2-yl)benzoyl chloride; lophine analogs;
D O I
10.1016/S0378-4347(98)00128-5
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A simple, rapid and highly sensitive high-performance liquid chromatographic method for the determination of methamphetamine and its metabolites in human urine was developed. Without tedious pretreatment procedures, aliquots of methamphetamine spiked or abusers urine samples were simply acidified, dried under a stream of N-2 gas, and then derivatized with 4-(4,5-diphenyl-1H-imidazol-2-yl)benzoyl chloride in acetonitrile in the presence of traces of triethylamine. Five derivatives were isocratically separated within 33 min by an ODS column, and the effluent was monitored at 440 nm (lambda(ex), 330 nm). Using 1-phenylethylamine as an internal standard calibration curves were confirmed to be linear up to at least 2 x 10(-5) M in urine with correlation coefficients of 0.998-1.000, while the detection limits at S/N = 3 were 0.6-5.2 fmol per 5-mu l injection. The relative standard deviations (n = 5) of inter- and intra-day variations were less than 8.9%. The correlation between the concentrations of methamphetamine and amphetamine in urine determined by the proposed method and another currently accepted one was satisfactory. The method was successfully applied to urine samples collected from methamphetamine addicts. (C) 1998 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:105 / 112
页数:8
相关论文
共 25 条
  • [1] Characterization of the fluorescence properties of 4-fluoro-7-nitrobenzo-2-oxa-1,3-diazole derivatives of some primary and secondary sympathomimetic amines
    Al-Dirbashi, O
    Kuroda, N
    Nakashima, K
    [J]. ANALYTICA CHIMICA ACTA, 1998, 365 (1-3) : 169 - 176
  • [2] High-performance liquid chromatography of methamphetamine and its related compounds in human urine following derivatization with fluorescein isothiocyanate
    AlDirbashi, O
    Kuroda, N
    Akiyama, S
    Nakashima, K
    [J]. JOURNAL OF CHROMATOGRAPHY B, 1997, 695 (02): : 251 - 258
  • [3] BUZAS A, 1950, B SOC CHIM FR, P139
  • [4] DESIGN AND DEVELOPMENT OF A SINGLE-REAGENT POLARIZATION FLUOROIMMUNOASSAY FOR METHAMPHETAMINE
    EREMIN, SA
    SCHIAVETTA, DE
    LOTEY, H
    SMITH, DS
    LANDON, J
    [J]. THERAPEUTIC DRUG MONITORING, 1988, 10 (03) : 327 - 332
  • [5] EREMIN SA, 1987, CLIN CHEM, V33, P1903
  • [6] GAN BK, 1991, J FORENSIC SCI, V36, P1331
  • [7] DETERMINATION OF AMPHETAMINE AND METHAMPHETAMINE IN BLOOD BY DERIVATIZATION WITH PERFLUOROOCTANOYL CHLORIDE AND GAS-CHROMATOGRAPHY MASS-SPECTROMETRY
    GJERDE, H
    HASVOLD, I
    PETTERSEN, G
    CHRISTOPHERSEN, AS
    [J]. JOURNAL OF ANALYTICAL TOXICOLOGY, 1993, 17 (02) : 65 - 68
  • [8] HAYAKAWA K, 1993, BIOL PHARM BULL, V16, P817
  • [9] DETERMINATION OF METHAMPHETAMINE, AMPHETAMINE AND PIPERIDINE IN HUMAN URINE BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH CHEMILUMINESCENCE DETECTION
    HAYAKAWA, K
    IMAIZUMI, N
    ISHIKURA, H
    MINOGAWA, E
    TAKAYAMA, N
    KOBAYASHI, H
    MIYAZAKI, M
    [J]. JOURNAL OF CHROMATOGRAPHY, 1990, 515 : 459 - 466
  • [10] HAYAKAWA K, 1989, J CHROMATOGR, V464, P343