Serum-mediated enhancement of TNF-α release by human monocytes stimulated with the yeast form of Candida albicans

被引：33

作者：

Ghezzi, MC ^{[1
]}

Raponi, G ^{[1
]}

Angeletti, S ^{[1
]}

Mancini, C ^{[1
]}

机构：

[1] Univ Rome La Sapienza, Fac Med, Chair Clin Microbiol 1, I-00185 Rome, Italy

来源：

JOURNAL OF INFECTIOUS DISEASES | 1998年 / 178卷 / 06期

关键词：

D O I：

10.1086/314484

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The role of serum in the production of tumor necrosis factor-alpha (TNF-alpha) by human monocytes stimulated with yeast-form Candida albicans was studied. Pre-exposure of C. albicans to human pooled serum enhanced both TNF-alpha mRNA and cytokine secretion compared with C, albicans preincubated with medium only. Serum factors involved were >30 kDa, were efficiently inhibited by D-mannose, and recognized both Ca++-dependent and -independent pathways, Preincubation of yeasts with rabbit mannose-binding protein (MBP) resulted in dose-related enhancement of TNF-alpha secretion, through a Ca++-dependent pathway inhibited by D-mannose, TNF-alpha levels were similarly induced in C. albicans preincubated with vitronectin and with serum. Ca++ depletion did not affect cytokine release, while D-mannose supplementation displayed inhibition. The latter effect was abolished after Ca++ depletion. These data call for an involvement of both MBP and vitronectin in the serum-mediated enhancement of TNF-alpha release upon stimulation of monocytes with yeast forms of C. albicans.

引用

页码：1743 / 1749

页数：7

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