Molecular basis of perhydrolase activity in serine hydrolases

被引：57

作者：

Bernhardt, P

Halt, K

Kazlauskas, RJ

机构：

[1] Univ Minnesota, Dept Biochem Mol Biol & Biophys, St Paul, MN 55108 USA

[2] Univ Minnesota, Ctr Microbial & Plant Genom, St Paul, MN 55108 USA

[3] AlbaNova Univ Ctr, Royal Inst Technol, Sch Biotechnol, Dept Biochem, S-10691 Stockholm, Sweden

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2005年 / 44卷 / 18期

关键词：

enzyme catalysis; hydrolases; molecular modeling; mutagenesis; peroxides;

D O I：

10.1002/anie.200463006

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

(Chemical Equation Presented) Changing substrates: A mutation that forms a cis-proline-peptide bond in a loop structure close to the active site of an aryl esterase from Pseudomonas fluorescens converts the enzyme into a perhydrolase (see picture). The switch in activity is explained by a new hydrogen bond formed between a backbone carbonyl oxygen atom and the peroxy deacylation intermediate. © 2005 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

收藏

页码：2742 / 2746

页数：5

相关论文

共 24 条

[1] LIPASE CATALYZED SYNTHESIS OF PEROXYCARBOXYLIC ACIDS AND LIPASE MEDIATED OXIDATIONS [J].

BJORKLING, F ;

FRYKMAN, H ;

GODTFREDSEN, SE ;

KIRK, O .

TETRAHEDRON, 1992, 48 (22) :4587-4592

[2]

Bornscheuer U. T., 2004, ANGEW CHEM, V116, P6156

[3] Catalytic promiscuity in biocatalysis: Using old enzymes to form new bonds and follow new pathways [J].

Bornscheuer, UT ;

Kazlauskas, RJ .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2004, 43 (45) :6032-6040

[4] Diverse catalytic activities in the αβ-hydrolase family of enzymes:: activation of H2O, HCN, H2O2, and O2 [J].

Bugg, TDH .

BIOORGANIC CHEMISTRY, 2004, 32 (05) :367-375

[5] Structure of an aryl esterase from Pseudomonas fluorescens [J].

Cheeseman, JD ;

Tocilj, A ;

Park, S ;

Schrag, JD ;

Kazlauskas, RJ .

ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY, 2004, 60 :1237-1243

[6] RELATIONSHIPS BETWEEN APPARENT BINDING-ENERGIES MEASURED IN SITE-DIRECTED MUTAGENESIS EXPERIMENTS AND ENERGETICS OF BINDING AND CATALYSIS [J].

FERSHT, AR .

BIOCHEMISTRY, 1988, 27 (05) :1577-1580

[7] MECHANISM OF THE REACTION OF CHYMOTRYPSIN WITH PARA-NITROPHENYL ACETATE [J].

GUTFREUND, H ;

STURTEVANT, JM .

BIOCHEMICAL JOURNAL, 1956, 63 (04) :656-661

[8]

HAGER LP, 1966, J BIOL CHEM, V241, P1769

[9] Structural investigation of the cofactor-free chloroperoxidases [J].

Hofmann, B ;

Tölzer, S ;

Pelletier, I ;

Altenbuchner, J ;

van Pée, KH ;

Hecht, HJ .

JOURNAL OF MOLECULAR BIOLOGY, 1998, 279 (04) :889-900

[10] Cloning and biochemical characterization of Co2+-activated bromoperoxidase-esterase (perhydrolase) from Pseudomonas putida IF-3 strain [J].

Itoh, N ;

Kawanami, T ;

Liu, JQ ;

Dairi, T ;

Miyakoshi, M ;

Nitta, C ;

Kimoto, Y .

BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY, 2001, 1545 (1-2) :53-66