Simultaneous analysis of intact and desulfated glucosinolates with a porous graphitized carbon column

A porous graphitized column was evaluated for the qualitative analysis of glucosinolates and desulfoglucosinolates. Successful separations were achieved using 0.1% aqueous trifluoroacetic acid-acetonitrile (85:15, v/v) as eluent. The selectivity obtained by LC on Hypercarb S allows compounds with a slight hydrophobic structure difference (e.g., a difference of one CH2 unit between two glucosinolates) to be satisfactorily resolved. Excellent separation of the two diastereoisomers progoitrin and epiprogoitrin in anionic or neutral form was observed.