RETENTION OF SOME GLUCOSINOLATES IN ANION-EXCHANGE CHROMATOGRAPHY .2. QUALITATIVE STUDY ON A STYRENE-DIVINYLBENZENE COPOLYMERIC ANION-EXCHANGER

The retention and separation of glucosinolates, as organic anions, were studied on a styrene-divinyibenzene copolymeric anion exchanger under isocratic elution conditions. The effects of the nature and concentration of different inorganic and organic eluent anions, and the influence of acetonitrile addition to the aqueous buffered mobile phase are investigated. For the alkanesulfonate eluent anions, elution strength increases noticeably with alkyl chain length. The plots of capacity factors of glucosinolates versus the reciprocal of organic eluent ion concentration show good linearity. The values of the slope and y-intercept data confirm the importance of the reverse phase interactions with respect to the ion exchange interactions in the glucosinolate retention mechanism. Our results prove that the isocratic analysis of intact glucosinolates on a polymeric anion exchanger is a suitable and convenient method that should be used as a complement to the current ion pairing chromatographic method.