Characterisation of a novel high M(r) glutenin subunit encoded by chromosome 1D of bread wheat

被引：8

作者：

Buonocore, F ^{[1
]}

Hickman, DR ^{[1
]}

Caporale, C ^{[1
]}

Porceddu, E ^{[1
]}

Lafiandra, D ^{[1
]}

Tatham, AS ^{[1
]}

Shewry, PR ^{[1
]}

机构：

[1] UNIV BRISTOL,DEPT AGR SCI,ACR LONG ASHTON RES STN,BRISTOL BS18 9AF,AVON,ENGLAND

来源：

JOURNAL OF CEREAL SCIENCE | 1996年 / 23卷 / 01期

关键词：

high M(r) glutenin subunits;

D O I：

10.1006/jcrs.1996.0005

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

A high M(r) subunit of wheat glutenin (1Dx2.2*), having an unusually high M(r) (approximately 110 000), was purified using a combination of preparative isoelectric focusing and reversed-phase high performance liquid chromatography. Comparison of its N-terminal sequence and amino acid composition with those of the allelic subunit 1Dx2 indicate that its greater M(r) could be due to the presence of a larger central repetitive domain. Spectrofluorimetric and circular dichroism analyses suggest that an increase in the length of this domain does not affect the conformational properties of the subunit. (C) 1996 Academic Press Limited

引用

页码：55 / 60

页数：6

共 19 条

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