Stabilisation of self-assembled DNA crystals by triplex-directed photo-cross-linking

被引：34

作者：

Abdallah, Hatem O. ^{[1
]}

Ohayon, Yoel P. ^{[1
]}

Chandrasekaran, Arun Richard ^{[1
,2
]}

Sha, Ruojie ^{[1
]}

Fox, Keith R. ^{[3
]}

Brown, Tom ^{[4
]}

Rusling, David A. ^{[3
]}

Mao, Chengde ^{[5
]}

Seeman, Nadrian C. ^{[1
]}

机构：

[1] NYU, Dept Chem, New York, NY 10003 USA

[2] SUNY Albany, RNA Inst, Albany, NY 12222 USA

[3] Univ Southampton, Ctr Biol Sci, Southampton SO17 1BJ, Hants, England

[4] Univ Oxford, Dept Chem, Chem Res Lab, 12 Mansfield Rd, Oxford OX1 3QZ, England

[5] Purdue Univ, Dept Chem, W Lafayette, IN 47907 USA

来源：

CHEMICAL COMMUNICATIONS | 2016年 / 52卷 / 51期

基金：

美国国家科学基金会; 英国生物技术与生命科学研究理事会;

关键词：

DOUBLE-HELICAL DNA; NANOSTRUCTURE; JUNCTIONS; DESIGN;

D O I：

10.1039/c6cc03695c

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The tensegrity triangle is a robust DNA motif that can self-assemble to generate macroscopic three-dimensional crystals. However, the stability of these crystals is dependent on the high ionic conditions used for crystal growth. Here we demonstrate that a triplex-forming oligonucleotide can be used to direct the specific intercalation, and subsequent photo-cross-linking, of 4,50,8-trimethylpsoralen to single or multiple loci within or between the tiles of the crystal. Cross-linking between the tiles of the crystal improves their thermal stability. Such an approach is likely to facilitate the removal of crystals from their mother liquor and may prove useful for applications that require greater crystal stability.

引用

页码：8014 / 8017

页数：4

共 16 条

[1] Targeting DNA with triplexes [J].