Simultaneous determination of six isoflavonoids in commercial Radix Astragali by HPLC-UV

被引：102

作者：

Wu, T

Bligh, SWA

Gu, LH

Wang, ZT ^{[1
]}

Liu, HP

Cheng, XM

Branford-White, CJ

Hu, ZB

机构：

[1] Shanghai R&D Ctr Standardizat Tradit Chinese Med, Shanghai 201203, Peoples R China

[2] London Metropolitan Univ, Inst Hlth Res & Policy, London N7 8DB, England

[3] Shanghai Univ Tradit Chinese Med, Inst Chinese Mat Med, Shanghai 201203, Peoples R China

[4] Anhui Coll Tradit Chinese Med, Hefei, Anhui Province, Peoples R China

来源：

FITOTERAPIA | 2005年 / 76卷 / 02期

关键词：

isoflavonoids; Radix Astragali; Astragalus membranaceus; A. membranaceus var. mongholicus; HPLC-UV; quantitative analysis;

D O I：

10.1016/j.fitote.2004.11.006

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

A HPLC-UV method for the quantification of six major isoflavonoids, calycosin 7-O-beta-D-glucoside (1), formononetin 7-O-beta-D-glucoside (2), (6 alpha R, 11 alpha R) 3-hydroxy-9,10-dimethoxypterocarpan-3-O-beta-D-glucoside (3), 7,2'-dihydroxy-3',4'-dimethoxyisoflavan-7-O-beta-D-glucoside (4), calycosin (5) and formononetin (6), in Radix Astragali (Huangqi) was developed and validated. The method was proven to be sensitive, specific, accurate and precise, as well as effective and easy. (c) 2005 Elsevier B.V. All rights reserved.

引用

页码：157 / 165

页数：9

共 20 条

[1] ASTRAGALOSIDES FROM HAIRY ROOT CULTURES OF ASTRAGALUS-MEMBRANACEUS .100. STUDIES ON PLANT-TISSUE CULTURES
HIROTANI, M
ZHOU, Y
LUI, H
FURUYA, T
[J]. PHYTOCHEMISTRY, 1994, 36 (03) : 665 - 670
[2] Kurashige S, 1999, CANCER INVEST, V17, P30
[3] The collagenolytic effects of the traditional Chinese medicine preparation, Han-Dan-Gan-Le, contribute to reversal of chemical-induced liver fibrosis in rats
Li, CX
Luo, J
Li, L
Cheng, ML
Huang, NH
Liu, J
Waalkes, MP
[J]. LIFE SCIENCES, 2003, 72 (14) : 1563 - 1571
[4] Liquid chromatography-electrospray ionization mass spectrometry study of the flavonoids of the roots of Astragalus mongholicus and A-membranaceus
Lin, LZ
He, XG
Lindenmaier, M
Nolan, G
Yang, J
Cleary, M
Qiu, SX
Cordell, GA
[J]. JOURNAL OF CHROMATOGRAPHY A, 2000, 876 (1-2) : 87 - 95
[5] Ma X. Q., 2000, Natural Medicines, V54, P213
[6] Chemical analysis of Radix Astragali (Huangqi) in China: A comparison with its adulterants and seasonal variations
Ma, XQ
Shi, Q
Duan, JA
Dong, TTX
Tsim, KWK
[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2002, 50 (17) : 4861 - 4866
[7] SHIMIZU N, 1991, CHEM PHARM BULL, V39, P2969, DOI 10.1248/cpb.39.2969
[8] Effect of astragali radix extract on lipopolysaccharide-induced inflammation in human amnion
Shon, YH
Kim, JH
Nam, KS
[J]. BIOLOGICAL & PHARMACEUTICAL BULLETIN, 2002, 25 (01) : 77 - 80
[9] ISOFLAVANS AND A PTEROCARPAN FROM ASTRAGALUS-MONGHOLICUS
SUBARNAS, A
OSHIMA, Y
HIKINO, H
[J]. PHYTOCHEMISTRY, 1991, 30 (08) : 2777 - 2780
[10] Inhibitory effects of Astragali Radix, a crude drug in Oriental medicines, on lipid peroxidation and protein oxidative modification by copper
Toda, S
Shirataki, Y
[J]. JOURNAL OF ETHNOPHARMACOLOGY, 1999, 68 (1-3) : 331 - 333

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