Identification of amygdalin and its major metabolites in rat urine by LC-MS/MS

被引：37

作者：

Ge, B. Y. ^{[1
]}

Chen, H. X. ^{[1
]}

Han, F. M. ^{[1
]}

Chen, Y. ^{[1
]}

机构：

[1] Hubei Univ, Coll Life Sci, Hubei Prov Key Lab Biotechnol Chinese Tradit Med, Wuhan 430062, Peoples R China

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2007年 / 857卷 / 02期

关键词：

amygdalin; LC-MS/MS; metabolite; rat urine;

D O I：

10.1016/j.jchromb.2007.07.036

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Amygdalin and its metabolites in rat urine were identified using liquid chromatography-electrospray ionization (ESI) tandem ion-trap mass spectrometry. The purified rat urine sample was separated using a reversed-phase C18 column with 10mM sodium phosphate buffer (pH 3.1) containing 30% methanol as the mobile phase, amygdalin and its metabolites were detected by on-line mass detector in selected ion monitoring (SIM) mode. The identification of the metabolites and elucidation of their structure were performed by comparing the changes in molecular masses (Delta M), retention times and MS2 spectral patterns of metabolites with those of parent drug. At least seven metabolites and the parent drug were found in rat urine after i.v. injection of 100 mg/kg doses of amygdalin. Among them, six metabolites were reported for the first time. (c) 2007 Elsevier B.V. All rights reserved.

引用

页码：281 / 286

页数：6

共 23 条

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