Rapid detection of vancomycin-resistant enterococci from rectal swabs by the Cepheid Xpert vanA/vanB assay

被引：30

作者：

Bourdon, Nancy

Berenger, Raphael

Lepoultier, Romain

Mouet, Audrey

Lesteven, Claire

Borgey, France

Fines-Guyon, Marguerite

Leclercq, Roland

Cattoir, Vincent

机构：

[1] CHU Cote Nacre, Microbiol Serv, Ctr Natl Resistance Antibiot, Lab Associe Enterocoques, F-14033 Caen, France

[2] CHU Cote Nacre, Serv Hyg Hosp, F-14033 Caen, France

[3] CHU Cote Nacre, Reseau Reg Hyg, F-14033 Caen, France

来源：

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2010年 / 67卷 / 03期

关键词：

Real-time PCR; GeneXpertg (R); VRE; vanA; vanB; STAPHYLOCOCCUS-AUREUS; METHICILLIN-RESISTANT; ENTEROVIRUS ASSAY; BLOOD CULTURES; IDENTIFICATION; MRSA; SPECIMENS; VANB;

D O I：

10.1016/j.diagmicrobio.2010.02.009

中图分类号：

R51 [传染病];

学科分类号：

100201 [内科学];

摘要：

The detection of vancomycin-resistant enterococci using a novel commercial multiplex real-time polymerase chain reaction assay (Xpert (TM) vanA/vanB, Cepheid, Sunnyvale, CA) was evaluated on 804 rectal swab specimens. Compared to enriched culture, sensitivity and negative predictive value of this method were 100%. Many false-positive results were recovered (sensitivity, 85.4%; positive predictive value, 8.7%), especially for the vanB gene. (C) 2010 Elsevier Inc. All rights reserved.

引用

页码：291 / 293

页数：3

共 26 条

[1]

Vancomycin resistance in gram-positive cocci [J].

Courvalin, P .

CLINICAL INFECTIOUS DISEASES, 2006, 42 :S25-S34

[2]

Detection of the van alphabet and identification of enterococci and staphylococci at the species level by multiplex PCR [J].

Depardieu, F ;

Perichon, B ;

Courvalin, P .

JOURNAL OF CLINICAL MICROBIOLOGY, 2004, 42 (12) :5857-5860

[3]

High prevalence of glycopeptide resistance genes vanB, vanD, and vanG not associated with enterococci in human fecal flora [J].

Domingo, MC ;

Huletsky, A ;

Giroux, R ;

Boissinot, K ;

Picard, FJ ;

Lebel, P ;

Ferraro, MJ ;

Bergeron, MG .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2005, 49 (11) :4784-4786

[4]

Characterization of a Tn5382-like transposon containing the vanB2 gene cluster in a Clostridium strain isolated from human faeces [J].

Domingo, MC ;

Huletsky, A ;

Bernal, A ;

Giroux, R ;

Boudreau, DK ;

Picard, FJ ;

Bergeron, MG .

JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2005, 55 (04) :466-474

[5]

Diagnostic Accuracy of a Rapid Real-Time Polymerase Chain Reaction Assay for Universal Intrapartum Group B Streptococcus Screening [J].

El Helali, Najoua ;

Nguyen, Jean-Claude ;

Ly, Aicha ;

Giovangrandi, Yves ;

Trinquart, Ludovic .

CLINICAL INFECTIOUS DISEASES, 2009, 49 (03) :417-423

[6]

A comparison of a new rapid real-time polymerase chain reaction system to traditional culture in determining group B streptococcus colonization [J].

Gavino, Michael ;

Wang, Eileen .

AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY, 2007, 197 (04) :388.e1-388.e4

[7]

Comparison of a Commercial Multiplex Real-Time PCR to the Cell Cytotoxicity Neutralization Assay for Diagnosis of Clostridium difficile Infections [J].

Huang, Haihui ;

Weintraub, Andrej ;

Fang, Hong ;

Nord, Carl Erik .

JOURNAL OF CLINICAL MICROBIOLOGY, 2009, 47 (11) :3729-3731

[8]

Comparison of the Xpert Methicillin-Resistant Staphylococcus aureus (MRSA) Assay, BD GeneOhm MRSA Assay, and Culture for Detection of Nasal and Cutaneous Groin Colonization by MRSA [J].

Kelley, P. G. ;

Grabsch, E. A. ;

Howden, B. P. ;

Gao, W. ;

Grayson, M. L. .

JOURNAL OF CLINICAL MICROBIOLOGY, 2009, 47 (11) :3769-3772

[9]

Multicenter beta trial of the GeneXpert enterovirus assay [J].

Kost, Christine B. ;

Rogers, Beverly ;

Oberste, M. Steven ;

Robinson, Christine ;

Eaves, Brenda L. ;

Leos, Kristi ;

Danielson, Susan ;

Satya, Malini ;

Weir, Fred ;

Nolte, Frederick S. .

JOURNAL OF CLINICAL MICROBIOLOGY, 2007, 45 (04) :1081-1086

[10]

Epidemiological and resistance issues in multidrug-resistant staphylococci and enterococci [J].

Leclercq, R. .

CLINICAL MICROBIOLOGY AND INFECTION, 2009, 15 (03) :224-231

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