An evaluation of molecular diagnostic tools for the detection and differentiation of human-pathogenic Cryptosporidium spp.

被引：39

作者：

Jiang, JL ^{[1
]}

Xiao, LH ^{[1
]}

机构：

[1] Ctr Dis Control & Prevent, Div Parasit Dis, Natl Ctr Infect Dis, Atlanta, GA 30341 USA

来源：

JOURNAL OF EUKARYOTIC MICROBIOLOGY | 2003年 / 50卷

关键词：

D O I：

10.1111/j.1550-7408.2003.tb00623.x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The performance of 10 commonly used genotyping tools in the detection and differentiation of 7 human-pathogenic Cryptosporidium spp. (C. hominis, C. parvum, C. meleagridis, C. felis, C. canis, C. muris and Cryptosporidium pig genotype 1) was evaluated. All 3 SU rRNA gene-based tools could amplify the DNA of 7 Cryptosporidium spp. efficiently. However. the tools based on the antigens TRAP-C 1, TRAP-C2 and COWP genes, the housekeeping genes HSP70 and DHFR, or a genomic sequence, failed to detect the DNA of C. felis, C. canis, Cryptosporidium pig genotype I, and C. muris. With the exception of I tool based on the TRAP-C2 gene, the PCR-RFLP or the PCR sequencing tools evaluated in this study could differentiate C. hominis, C. parvum and C. meleagridis from each other, and 2 SSU rRNA gene-based tools could differentiate all 7 Cryptosporidium spp. Thus, a thorough understanding of the strength and weakness of each technique is needed when using molecular diagnostic tool in epidemiological investigations of human cryptosporidiosis.

引用

页码：542 / 547

页数：6

共 14 条

[1] Modification of a rapid method for the identification of gene-specific polymorphisms in Cryptosporidium parvum and its application to clinical and epidemiological investigations [J].

Elwin, K ;

Chalmers, RM ;

Roberts, R ;

Guy, EC ;

Casemore, DP .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2001, 67 (12) :5581-5584

[2] Cryptosporidium muris infection in an HIV-infected adult, Kenya [J].

Gatei, W ;

Ashford, RW ;

Beeching, NJ ;

Kamwati, SK ;

Greensill, J ;

Hart, CA .

EMERGING INFECTIOUS DISEASES, 2002, 8 (02) :204-206

[3]

Gibbons Cindy L., 1998, Parasitology International, V47, P139, DOI 10.1016/S1383-5769(98)00012-9

[4] Sensitive genotyping of Cryptosporidium parvum by PCR-RFLP analysis of the 70-kilodalton heat shock protein (HSP70) gene [J].

Gobet, P ;

Toze, S .

FEMS MICROBIOLOGY LETTERS, 2001, 200 (01) :37-41

[5] PCR-restriction fragment length polymorphism analysis of a diagnostic 452-base-pair DNA fragment discriminates between Cryptosporidium parvum and C-meleagridis and between C-parvum isolates of human and animal origin [J].

Guyot, K ;

Follet-Dumoulin, A ;

Recourt, C ;

Lelièvre, E ;

Cailliez, JC ;

Dei-Cas, E .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2002, 68 (04) :2071-2076

[6] Characterization of Cryptosporidium parvum in human and animal feces by single-tube nested polymerase chain reaction and restriction analysis [J].

Homan, W ;

van Gorkom, T ;

Kan, YY ;

Hepener, J .

PARASITOLOGY RESEARCH, 1999, 85 (8-9) :707-712

[7] Novel Cryptosporidium genotypes in sporadic cryptosporidiosis cases:: First report of human infections with a cervine genotype [J].

Ong, CSL ;

Eisler, DL ;

Alikhani, A ;

Fung, VWK ;

Tomblin, J ;

Bowie, WR ;

Isaac-Renton, JL .

EMERGING INFECTIOUS DISEASES, 2002, 8 (03) :263-268

[8] Nested polymerase chain reaction for amplification of the Cryptosporidium oocyst wall protein gene [J].

Pedraza-Díaz, S ;

Amar, C ;

Nichols, GL ;

McLauchlin, J .

EMERGING INFECTIOUS DISEASES, 2001, 7 (01) :49-56

[9] Cryptosporidium parvum:: PCR-RFLP analysis of the TRAP-C1 (thrombospondin-related adhesive protein of Cryptosporidium-1) gene discriminates between two alleles differentially associated with parasite isolates of animal and human origin [J].

Spano, F ;

Putignani, L ;

Guida, S ;

Crisanti, A .

EXPERIMENTAL PARASITOLOGY, 1998, 90 (02) :195-198

[10] Species-specific, nested PCR-restriction fragment length polymorphism detection of single Cryptosporidium parvum oocysts [J].

Sturbaum, GD ;

Reed, C ;

Hoover, PJ ;

Jost, BH ;

Marshall, MM ;

Sterling, CR .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2001, 67 (06) :2665-2668

← 1 2 →