Development of a novel method combining HPLC fingerprint and multi-ingredients quantitative analysis for quality evaluation of traditional chinese medicine preparation

被引：158

作者：

Yang, Dong-Zhi ^{[1
,2
]}

An, Ti Qiang ^{[2
]}

Jiang, Xiang-Lan ^{[1
]}

Tang, Dao-Quan ^{[1
,2
]}

Gao, Yuan-Yuan ^{[1
]}

Zhao, Hong-Tao ^{[3
]}

Wu, Xiao-Wen ^{[3
]}

机构：

[1] Xuzhou Med Coll, Dept Pharmaceut Anal, Xuzhou Jiangsu 221004, Peoples R China

[2] Xuzhou Med Coll, Key Lab New Drug & Clin Applicat, Xuzhou Jiangsu 221004, Peoples R China

[3] Xuzhou Med Coll, Sch Pharm, Xuzhou Jiangsu 221004, Peoples R China

来源：

TALANTA | 2011年 / 85卷 / 02期

关键词：

Fingerprint analysis; Quality control; Multi-ingredient quantitative analysis; Shuang-huang-lian (SHL) oral liquid; High-performance liquid chromatography (HPLC); LIAN ORAL LIQUID; CHROMATOGRAPHIC FINGERPRINT; CHLOROGENIC ACID; HERBAL MEDICINES; LC; CONSTITUENTS;

D O I：

10.1016/j.talanta.2011.04.059

中图分类号：

O65 [分析化学];

学科分类号：

070302 [分析化学];

摘要：

A novel method combining high performance liquid chromatography (HPLC) fingerprint and simultanous quantitative analysis of multiple acitve components was developed and validated for quality evaluation of one type of traditional Chinese medicine preparations: Shuang-huang-lian (SHL) oral liquid formulation. For fingerprint analysis, 45 peaks were selected as the common peaks to evaluate the similarities among several different SHL oral liquid preparations collected from manufacturers. Additionally, simultanous quantification of eleven markers, including chlorogenic acid, caffeic acid, rutin, forsythiaside, scutellarin, baicalin, forsythin, luteoloside, apigenin, baicalein and wogonin, was performed. Statistical analysis of the obtained data demonstrated that our method has achieved desired linearity, precision and accuracy. Finally, concentrations of these eleven markers in SHL oral liquid prepared by different manufacturers in China were determined. These results demonstrated that the combination of HPLC chromatographic fingerprint and simultaneous quantification of multi-ingredients offers an efficient and reliable approach for quality evaluation of SHL oral liquid preparations. (C) 2011 Elsevier B.V. All rights reserved.

引用

页码：885 / 890

页数：6

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