Influence of the organic solvents on the activity in water and the conformation of Candida rugosa lipase: Description of a lipase-activating pretreatment

被引：36

作者：

Torres, C ^{[1
]}

Otero, C ^{[1
]}

机构：

[1] CSIC, INST CATALISIS, E-28049 MADRID, SPAIN

来源：

ENZYME AND MICROBIAL TECHNOLOGY | 1996年 / 19卷 / 08期

关键词：

Candida cylindracea lipase; Candida rugosa; lipase; interfacial activation; fluorescence; enzymes in organic solvents;

D O I：

10.1016/S0141-0229(97)82686-5

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The influence on lipase activity in water of a pretreatment on Candida rugosa lipase using water miscible and immiscible solvents was studied. The lipase activity in the hydrolysis of esteric substrates in aqueous media increases when the lipase was previously treated with various nearly anhydrous organic media. This activation, which was irreversible, was higher for longer pretreatment times. It was dependent on the pretreatment medium (water activity and solvent used). A relation between variations in the emission intensity and the activities of created and untreated lipases was found. Activating pretreatment did not shift the peak of fluorescence emission but gave rise to variations in the secondary protein structure by increasing the helical nature. A similar increment in the hydrolysis rate in water can be obtained with the addition of an appropriate amount of solvent (acetonitrile or n-heptane) to the aqueous reaction medium. (C) 1996 by Elsevier Science Inc.

引用

页码：594 / 600

页数：7

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