thermo-msf-parser: An Open Source Java']Java Library to Parse and Visualize Thermo Proteome Discoverer msf Files

被引:36
作者
Colaert, Niklaas [1 ,2 ]
Barsnes, Harald [3 ,4 ]
Vaudel, Marc [5 ]
Helsens, Kenny [1 ,2 ]
Timmerman, Evy [1 ,2 ]
Sickmann, Albert [5 ,6 ]
Gevaert, Kris [1 ,2 ]
Martens, Lennart [1 ,2 ]
机构
[1] VIB, Dept Med Prot Res, Ghent, Belgium
[2] Univ Ghent, Dept Biochem, B-9000 Ghent, Belgium
[3] Univ Bergen, Prote Unit, Dept Biomed, Bergen, Norway
[4] Univ Bergen, Computat Biol Unit, Bergen, Norway
[5] Leibniz Inst Analyt Wissensch ISAS eV, Dortmund, Germany
[6] Ruhr Univ Bochum, MPC, Bochum, Germany
基金
比利时弗兰德研究基金会;
关键词
proteomics; bioinformatics; mass spectrometry; MASS-SPECTROMETRY DATA; QUANTIFICATION; IDENTIFICATION; SOFTWARE; PEPTIDE; TANDEM; TOOL;
D O I
10.1021/pr2005154
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The Thermo Proteome Discoverer program integrates both peptide identification and quantification into a single workflow for peptide-centric proteomics. Furthermore, its close integration with Thermo mass spectrometers has made it increasingly popular in the field. Here, we present a Java library to parse the msf files that constitute the output of Proteome Discoverer. The parser is also implemented as a graphical user interface allowing convenient access to the information found in the msf files, and in Rover, a program to analyze and validate quantitative proteomics information. All code, binaries, and documentation is freely available at http://thermo-msf-parser.googlecode.com.
引用
收藏
页码:3840 / 3843
页数:4
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