Electrochromatography in microchips packed with conventional reversed-phase silica particles

被引：21

作者：

Gaspar, Attila ^{[1
,2
]}

Hernandez, Lilia ^{[2
]}

Stevens, Schetema ^{[2
]}

Gomez, Frank A. ^{[2
]}

机构：

[1] Univ Debrecen, Dept Inorgan & Analyt Chem, H-4032 Debrecen, Hungary

[2] Calif State Univ Los Angeles, Dept Chem & Biochem, Los Angeles, CA 90032 USA

来源：

ELECTROPHORESIS | 2008年 / 29卷 / 08期

关键词：

chip; electrochromatography; pressure injection; reversed-phase silica particles;

D O I：

10.1002/elps.200700489

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

This paper shows the applicability of a disposable and inexpensive microfluidic chip for electrochromatographic separations. The chip, recently developed by us for chip-based LC, was fabricated from PDMS incorporating conventional chromatographic RP silica particles (C18) without the use of frits. Three cephalosporin antibiotics were used to demonstrate the applicability of the chip-based chromatographic packing for electrochromatographic determinations. The used sample injection method utilizes hydrodynamic pressure, thereby, reducing the propensity for sample bias during the injection.

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收藏

页码：1638 / 1642

页数：5

相关论文

共 23 条

[1] Diamond microchips for fast chromatography of proteins [J].

Björkman, H ;

Ericson, C ;

Hjertén, S ;

Hjort, K .

SENSORS AND ACTUATORS B-CHEMICAL, 2001, 79 (01) :71-77

[2] An integrated fritless column for on-chip capillary electrochromatography with conventional stationary phases [J].

Ceriotti, L ;

de Rooij, NF ;

Verpoorte, E .

ANALYTICAL CHEMISTRY, 2002, 74 (03) :639-647

[3] On-chip chromatography: the last twenty years [J].

de Mello, A .

LAB ON A CHIP, 2002, 2 (03) :48N-54N

[4] Micro total analysis systems. Latest advancements and trends [J].

Dittrich, Petra S. ;

Tachikawa, Kaoru ;

Manz, Andreas .

ANALYTICAL CHEMISTRY, 2006, 78 (12) :3887-3907

[5] Rapid prototyping of microfluidic systems in poly(dimethylsiloxane) [J].

Duffy, DC ;

McDonald, JC ;

Schueller, OJA ;

Whitesides, GM .

ANALYTICAL CHEMISTRY, 1998, 70 (23) :4974-4984

[6] Electroosmosis- and pressure-driven chromatography in chips using continuous beds [J].

Ericson, C ;

Holm, J ;

Ericson, T ;

Hjertén, S .

ANALYTICAL CHEMISTRY, 2000, 72 (01) :81-87

[7] Fabrication of fritless chromatographic microchips packed with conventional reversed-phase silica particles [J].

Gaspar, Attila ;

Piyasena, Menake E. ;

Gomez, Frank A. .

ANALYTICAL CHEMISTRY, 2007, 79 (20) :7906-7909

[8] Fabrication of nanocolumns for liquid chromatography [J].

He, B ;

Tait, N ;

Regnier, F .

ANALYTICAL CHEMISTRY, 1998, 70 (18) :3790-3797

[9] OPEN-CHANNEL ELECTROCHROMATOGRAPHY ON A MICROCHIP [J].

JACOBSON, SC ;

HERGENRODER, R ;

KOUTNY, LB ;

RAMSEY, JM .

ANALYTICAL CHEMISTRY, 1994, 66 (14) :2369-2373

[10] Pressure injection on a valved microdevice for electrophoretic analysis of submicroliter samples [J].

Karlinsey, JM ;

Monahan, J ;

Marchiarullo, DJ ;

Ferrance, JP ;

Landers, JP .

ANALYTICAL CHEMISTRY, 2005, 77 (11) :3637-3643