Electrochemical biosensors utilising electron transfer in heme proteins immobilised on Fe3O4 nanoparticles

被引：136

作者：

Cao, DF ^{[1
]}

He, PL ^{[1
]}

Hu, NF ^{[1
]}

机构：

[1] Beijing Normal Univ, Dept Chem, Beijing 100875, Peoples R China

来源：

ANALYST | 2003年 / 128卷 / 10期

关键词：

D O I：

10.1039/b308242c

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Fe3O4 nanoparticles cast on pyrolytic graphite (PG) electrodes were used to immobilize hemoglobin (Hb), myoglobin (Mb) and horseradish peroxidase (HRP). The Fe3O4 nanoparticles provided a favorable microenvironment for the proteins to directly transfer electrons with electrodes. The protein-Fe3O4 films were used to electrochemically catalyze the reduction of oxygen, trichloroacetic acid, nitrite and hydrogen peroxide, and showed a potential applicability in fabricating biosensors. Transmission electron microscopy (TEM), UV-visible absorption and reflectance absorption infrared (RAIR) spectroscopy, and cyclic and square wave voltammetry, were used to characterize the films.

引用

页码：1268 / 1274

页数：7

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