Electrochemical biosensors utilising electron transfer in heme proteins immobilised on Fe3O4 nanoparticles

被引：136

作者：

Cao, DF ^{[1
]}

He, PL ^{[1
]}

Hu, NF ^{[1
]}

机构：

[1] Beijing Normal Univ, Dept Chem, Beijing 100875, Peoples R China

来源：

ANALYST | 2003年 / 128卷 / 10期

关键词：

D O I：

10.1039/b308242c

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Fe3O4 nanoparticles cast on pyrolytic graphite (PG) electrodes were used to immobilize hemoglobin (Hb), myoglobin (Mb) and horseradish peroxidase (HRP). The Fe3O4 nanoparticles provided a favorable microenvironment for the proteins to directly transfer electrons with electrodes. The protein-Fe3O4 films were used to electrochemically catalyze the reduction of oxygen, trichloroacetic acid, nitrite and hydrogen peroxide, and showed a potential applicability in fabricating biosensors. Transmission electron microscopy (TEM), UV-visible absorption and reflectance absorption infrared (RAIR) spectroscopy, and cyclic and square wave voltammetry, were used to characterize the films.

引用

页码：1268 / 1274

页数：7

共 57 条

[21]

KUMOSINSKI TF, 1994, MOL MODELING, P71

[22] Nitrite reduction by myoglobin in surfactant films [J].

Lin, R ;

Bayachou, M ;

Greaves, J ;

Farmer, PJ .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1997, 119 (51) :12689-12690

[23] Heme protein-gluten films: voltammetric studies and their electrocatalytic properties [J].

Liu, HY ;

Hu, NF .

ANALYTICA CHIMICA ACTA, 2003, 481 (01) :91-99

[24] Direct voltammetry and electrocatalytic properties of catalase incorporated in polyacrylamide hydrogel films [J].

Lu, HY ;

Li, Z ;

Hu, NF .

BIOPHYSICAL CHEMISTRY, 2003, 104 (03) :623-632

[25] Films of manganese oxide nanoparticles with polycations or myoglobin from alternate-layer adsorption [J].

Lvov, Y ;

Munge, B ;

Giraldo, O ;

Ichinose, I ;

Suib, SL ;

Rusling, JF .

LANGMUIR, 2000, 16 (23) :8850-8857

[26] Electroactive myoglobin films grown layer-by-layer with poly(styrenesulfonate) on pyrolytic graphite electrodes [J].

Ma, HY ;

Hu, NF ;

Rusling, JF .

LANGMUIR, 2000, 16 (11) :4969-4975

[27] ELECTROSTATIC EFFECTS IN HEMOGLOBIN - HYDROGEN-ION EQUILIBRIA IN HUMAN DEOXYHEMOGLOBIN AND OXYHEMOGLOBIN-A .104. [J].

MATTHEW, JB ;

HANANIA, GIH ;

GURD, FRN .

BIOCHEMISTRY, 1979, 18 (10) :1919-1928

[28] Direct binding of protein to magnetic particles [J].

Mehta, RV ;

Upadhyay, RV ;

Charles, SW ;

Ramchand, CN .

BIOTECHNOLOGY TECHNIQUES, 1997, 11 (07) :493-496

[29]

MEITES L, 1965, POLAROGRAPHIC TECHNI, P282

[30] Optimization of long-term stability of magnetic fluids from magnetite and synthetic polyelectrolytes [J].

Mendenhall, GD ;

Geng, YP ;

Hwang, J .

JOURNAL OF COLLOID AND INTERFACE SCIENCE, 1996, 184 (02) :519-526

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