An immune response to ice crystals in North Atlantic fishes

被引:13
作者
Verdier, JM
Ewart, KV
Griffith, M
Hew, CL
机构
[1] UNIV TORONTO,DEPT CLIN BIOCHEM,TORONTO,ON M5G 1L5,CANADA
[2] INSERM,U315,F-13258 MARSEILLE,FRANCE
[3] HOSP SICK CHILDREN,RES INST,TORONTO,ON M5G 1X8,CANADA
[4] UNIV TORONTO,DEPT BIOCHEM,TORONTO,ON M5G 1L5,CANADA
[5] UNIV WATERLOO,DEPT BIOL,WATERLOO,ON N2L 3G1,CANADA
来源
EUROPEAN JOURNAL OF BIOCHEMISTRY | 1996年 / 241卷 / 03期
关键词
ice-nucleating activity; ice-binding immunoglobulin; freeze-resistant fish; antifreeze protein;
D O I
10.1111/j.1432-1033.1996.00740.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In mammals, the presence of crystals composed of small organic molecules, including urate and related compounds, has been shown to trigger an inflammatory response and the subsequent production of specific immunoglobulins (Ig's). Many fishes that are exposed to ice crystals in cold temperate and polar oceans may harbour ice crystals internally. Here, we report evidence for a specific immune response to ice crystals in cold-ocean marine fishes. Using ice nucleation activity as an assay anti-ice Ig's were detected in the sera of the cold-ocean marine fish species. ocean pout (Macrozoarces, americanus) and Atlantic herring (Clupea harengus harengus), but not in the sera of species that are not exposed to ice. Purified Ig's isolated from ocean pout serum using two different protocols showed ice nucleation activity, thus demonstrating the presence of ice binding specificity among these Ig's.
引用
收藏
页码:740 / 743
页数:4
相关论文
共 25 条
[11]   A MODEL OF THE 3-DIMENSIONAL STRUCTURE OF ICE NUCLEATION PROTEINS [J].
KAJAVA, AV ;
LINDOW, SE .
JOURNAL OF MOLECULAR BIOLOGY, 1993, 232 (03) :709-717
[12]   ANTIBODIES AGAINST CRYSTALS [J].
KAM, M ;
PERLTREVES, D ;
CASPI, D ;
ADDADI, L .
FASEB JOURNAL, 1992, 6 (08) :2608-2613
[13]  
Kam Michal, 1994, Journal of Molecular Recognition, V7, P257, DOI 10.1002/jmr.300070404
[14]   CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].
LAEMMLI, UK .
NATURE, 1970, 227 (5259) :680-+
[15]   MOLECULAR RECOGNITION IN BIOMINERALIZATION [J].
MANN, S .
NATURE, 1988, 332 (6160) :119-124
[16]   INTERACTIONS BETWEEN ACIDIC MATRIX MACROMOLECULES AND CALCIUM-PHOSPHATE ESTER CRYSTALS - RELEVANCE TO CARBONATE APATITE FORMATION IN BIOMINERALIZATION [J].
MORADIANOLDAK, J ;
FROLOW, F ;
ADDADI, L ;
WEINER, S .
PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES, 1992, 247 (1318) :47-55
[17]   CATALYTIC ANTIBODIES - PERUSING COMBINATORIAL LIBRARIES [J].
POSNER, B ;
SMILEY, J ;
LEE, I ;
BENKOVIC, S .
TRENDS IN BIOCHEMICAL SCIENCES, 1994, 19 (04) :145-150
[18]   DIFFERENCES IN MUCUS AND SERUM IMMUNOGLOBULIN OF CARP (CYPRINUS-CARPIO L) [J].
ROMBOUT, JHWM ;
TAVERNE, N ;
VANDEKAMP, M ;
TAVERNETHIELE, AJ .
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY, 1993, 17 (04) :309-317
[19]  
VALERIO PF, 1992, J EXP BIOL, V164, P135
[20]  
VALI G, 1971, J ATMOS SCI, V28, P402, DOI 10.1175/1520-0469(1971)028<0402:QEOERA>2.0.CO