Rat Hsp20 confers thermoresistance in a clonal survival assay, but fails to protect coexpressed luciferase in Chinese hamster ovary cells

被引：21

作者：

van de Klundert, FAJM ^{[1
]}

van den Ijssel, PRLA ^{[1
]}

Stege, GJJ ^{[1
]}

de Jong, WW ^{[1
]}

机构：

[1] Catholic Univ Nijmegen, Dept Biochem, NL-6500 HB Nijmegen, Netherlands

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1999年 / 254卷 / 01期

关键词：

small heat shock protein; clonal survival assay; luciferase;

D O I：

10.1006/bbrc.1998.9917

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Hsp20 is a mammalian small heat shock protein with some deviating in vitro characteristics. We now compare the in vivo cellular thermoprotective abilities of Hsp20 with those of its direct relative, alpha beta-crystallin. In a clonal survival assay Chinese hamster ovary (CHO) cells stably overexpressing Hsp20 survive equally well as alpha beta-crystallin-expressing cells, after a heat shock. In a transient assay, however, overexpression of Hsp20 did not result in an enhanced recovery of coexpressed firefly luciferase after heat shock, in contrast to alpha beta-crystallin. This might indicate that these highly homologous stress proteins are involved in at least partially distinct protective activities in cultured cells. (C) 1999 Academic Press.

引用

页码：164 / 168

页数：5

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