Hard X-ray fluorescence tomography - an emerging tool for structural visualization

被引：116

作者：

de Jonge, Martin D. ^{[1
]}

Vogt, Stefan ^{[2
]}

机构：

[1] Australian Synchrotron, Clayton, Vic 3168, Australia

[2] Argonne Natl Lab, Argonne, IL 61439 USA

来源：

CURRENT OPINION IN STRUCTURAL BIOLOGY | 2010年 / 20卷 / 05期

关键词：

PHASE-CONTRAST; SYNCHROTRON; RECONSTRUCTION; MICROPROBE; MICROTOMOGRAPHY; NANOTOMOGRAPHY; DETECTOR; WASTE; CELLS; MODEL;

D O I：

10.1016/j.sbi.2010.09.002

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Hard X-ray fluorescence microscopy is well-suited to in-situ investigations of trace metal distributions within whole, unstained, biological tissue, with sub-parts-per-million detection achievable in whole cells The high penetration of X-rays indicates the use of X-ray fluorescence tomography for structural visualization and recent measurements have realised sub-500-nm tomography on a 10-mu m cell Limitations of present approaches impact the duration of an experiment and imaging fidelity Developments in X-ray resolution, detector speed cryogenic environments and the incorporation of auxiliary signals are being pursued within the synchrotron community Several complementary approaches to X-ray fluorescence tomography will be routinely available to the biologist in the near future We discuss these approaches and review applications of biological relevance

引用

页码：606 / 614

页数：9