Cloning of cDNA for canine interleukin-18 and canine interleukin-1β converting enzyme and expression of canine interleukin-18

被引：12

作者：

Okano, F

Satoh, M

Ido, T

Yamada, K

机构：

[1] Toray Ind Inc, Chem Res Labs, Minato Ku, Nagoya, Aichi 4558502, Japan

[2] Toray Ind Inc, Basic Res Labs, Kanasawa, Japan

来源：

JOURNAL OF INTERFERON AND CYTOKINE RESEARCH | 1999年 / 19卷 / 01期

关键词：

D O I：

10.1089/107999099314388

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cloning of canine interleukin-18 (IL-18) and canine interleukin-1 beta converting enzyme (ICE) cDNA was carried out by using murine IL-18 cDNA and human ICE cDNA, respectively, as probes. Sequence homology to known sequences of human, mouse, or rat genes was noted at nucleotide and amino acid levels. Canine IL-18 mRNA was expressed in various canine organs, whereas canine ICE mRNA was expressed in only a few, particularly in the brain and testis. Cloned canine IL-18 cDNA was expressed in Escherichia coli, The resulting protein promoted induction of canine interferon-gamma (IFN-gamma) from stimulated canine lymphocytes, Canine IL-18 and canine IL-12 produced canine IFN-gamma synergistically. Canine IL-18 suppressed the growth of tumor cells transplanted in SCID mice. Cloned canine IL-18 should prove useful as an anticancer agent.

引用

页码：27 / 32

页数：6

共 15 条

[1] A newly defined interleukin-1? [J].

Bazan, JF ;

Timans, JC ;

Kastelein, RA .

NATURE, 1996, 379 (6566) :591-591

[2] MOLECULAR-CLONING OF THE INTERLEUKIN-1-BETA CONVERTING ENZYME [J].

CERRETTI, DP ;

KOZLOSKY, CJ ;

MOSLEY, B ;

NELSON, N ;

VANNESS, K ;

GREENSTREET, TA ;

MARCH, CJ ;

KRONHEIM, SR ;

DRUCK, T ;

CANNIZZARO, LA ;

HUEBNER, K ;

BLACK, RA .

SCIENCE, 1992, 256 (5053) :97-100

[3] Interferon-gamma-inducing factor, a novel cytokine, enhances Fas ligand-mediated cytotoxicity of murine T helper 1 cells [J].

Dao, T ;

Ohashi, K ;

Kayano, T ;

Kurimoto, M ;

Okamura, H .

CELLULAR IMMUNOLOGY, 1996, 173 (02) :230-235

[4] Caspase-1 processes IFN-gamma-inducing factor and regulates LPS-induced IFN-gamma production [J].

Ghayur, T ;

Banerjee, S ;

Hugunin, M ;

Butler, D ;

Herzog, L ;

Carter, A ;

Quintal, L ;

Sekut, L ;

Talanian, R ;

Paskind, M ;

Wong, W ;

Kamen, R ;

Tracey, D ;

Allen, H .

NATURE, 1997, 386 (6625) :619-623

[5] Activation of interferon-gamma inducing factor mediated by interleukin-1 beta converting enzyme [J].

Gu, Y ;

Kuida, K ;

Tsutsui, H ;

Ku, G ;

Hsiao, K ;

Fleming, MA ;

Hayashi, N ;

Higashino, K ;

Okamura, H ;

Nakanishi, K ;

Kurimoto, M ;

Tanimoto, T ;

Flavell, RA ;

Sato, V ;

Harding, MW ;

Livingston, DJ ;

Su, MSS .

SCIENCE, 1997, 275 (5297) :206-209

[6]

Kohno K, 1997, J IMMUNOL, V158, P1541

[7] THE FAS DEATH FACTOR [J].

NAGATA, S ;

GOLSTEIN, P .

SCIENCE, 1995, 267 (5203) :1449-1456

[8] CLONING OF A NEW CYTOKINE THAT INDUCES IFN-GAMMA PRODUCTION BY T-CELLS [J].

OKAMURA, H ;

TSUTSUI, H ;

KOMATSU, T ;

YUTSUDO, M ;

HAKURA, A ;

TANIMOTO, T ;

TORIGOE, K ;

OKURA, T ;

NUKADA, Y ;

HATTORI, K ;

AKITA, K ;

NAMBA, M ;

TANABE, F ;

KONISHI, K ;

FUKUDA, S ;

KURIMOTO, M .

NATURE, 1995, 378 (6552) :88-91

[9] Cloning and expression of the cDNA for canine interleukin-12 [J].

Okano, F ;

Satoh, M ;

Yamada, K .

JOURNAL OF INTERFERON AND CYTOKINE RESEARCH, 1997, 17 (11) :713-718

[10] Defective NK cell activity and Th1 response in IL-18-deficient mice [J].

Takeda, K ;

Tsutsui, H ;

Yoshimoto, T ;

Adachi, O ;

Yoshida, N ;

Kishimoto, T ;

Okamura, H ;

Nakanishi, K ;

Akira, S .

IMMUNITY, 1998, 8 (03) :383-390

← 1 2 →