Detection of 210 kDa receptor protein for a leaf-movement factor by using novel photoaffinity probes

被引：20

作者：

Fujii, T ^{[1
]}

Manabe, Y ^{[1
]}

Sugimoto, T ^{[1
]}

Ueda, M ^{[1
]}

机构：

[1] Tohoku Univ, Dept Chem, Aoba Ku, Sendai, Miyagi 9808578, Japan

来源：

TETRAHEDRON | 2005年 / 61卷 / 33期

基金：

日本学术振兴会;

关键词：

photoaffinity labelling; receptor; potassium lespedezate;

D O I：

10.1016/j.tet.2005.06.022

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

Circadian rhythmic plant leaf-movement, called nyctinasty, is controlled by a time-course change in the internal concentration of the leaf-movement factor in the plant body. We revealed that specific binding proteins (210 and 180 kDa) for the leaf-movement factor, potassium lespedezate (1), are contained in the plasma membrane of the plant motor cell by using novel synthetic photoaffinity probes. These proteins are localized on the motor cell in the plant body, and would be potential receptors for the leaf-movement factor to control the leaf-movement. Our study is a rare successful result of the detection of membrane receptors by using a synthetic photoaffinity probe designed on a biologically active natural product. And these results also advance a guideline for probe design towards successful photoaffinity labeling. (c) 2005 Elsevier Ltd. All rights reserved.

引用

页码：7874 / 7893

页数：20

共 22 条

[1]

Darwin C., 1882, The Power of Movement in Plants

[2] Using photolabile ligands in drug discovery and development [J].

Dormán, G ;

Prestwich, GD .

TRENDS IN BIOTECHNOLOGY, 2000, 18 (02) :64-77

[3] BENZOPHENONE PHOTOPHORES IN BIOCHEMISTRY [J].

DORMAN, G ;

PRESTWICH, GD .

BIOCHEMISTRY, 1994, 33 (19) :5661-5673

[4] Synthesis of a novel bioactive photoaffinity probe based on a leaf-movement factor with potential high binding affinity to its receptor molecule [J].

Fujii, T ;

Sugimoto, T ;

Yamamura, S ;

Ueda, M .

TETRAHEDRON LETTERS, 2003, 44 (12) :2497-2499

[5]

Hatanaka Y, 1994, Bioorg Med Chem, V2, P1367, DOI 10.1016/S0968-0896(00)82088-X

[6] NOVEL FAMILY OF AROMATIC DIAZIRINES FOR PHOTOAFFINITY-LABELING [J].

HATANAKA, Y ;

HASHIMOTO, M ;

KURIHARA, H ;

NAKAYAMA, H ;

KANAOKA, Y .

JOURNAL OF ORGANIC CHEMISTRY, 1994, 59 (02) :383-387

[7] Understanding and controlling the cell cycle with natural products [J].

Hung, DT ;

Jamison, TF ;

Schreiber, SL .

CHEMISTRY & BIOLOGY, 1996, 3 (08) :623-639

[8] RECENT TRENDS IN PHOTOAFFINITY-LABELING [J].

KOTZYBAHIBERT, F ;

KAPFER, I ;

GOELDNER, M .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 1995, 34 (12) :1296-1312

[9] 120-and 160-kDa receptors for endogenous mitogenic peptide, phytosulfokine-α, in rice plasma membranes [J].

Matsubayashi, Y ;

Sakagami, Y .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (20) :15520-15525

[10] Fluorescence studies on nyctinasty which suggest the existence of genus-specific receptors for leaf-movement factor [J].

Nagano, H ;

Kato, E ;

Yamamura, S ;

Ueda, M .

ORGANIC & BIOMOLECULAR CHEMISTRY, 2003, 1 (18) :3186-3192

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