Proteomic Analysis of Four Brazilian MON810 Maize Varieties and Their Four Non-Genetically-Modified Isogenic Varieties

Profiling techniques have been suggested as a nontargeted approach to detect unintended,effects in genetically modified (GM) plants Seedlings from eight Brazilian maize varieties, four MON810 GM varieties and four non-GM isogenic varieties, were grown under controlled environmental conditions Physiological parameters (aerial part weight, main leaf length, and chlorophyll and total protein contents) were compared, and some differences were observed Nevertheless, these differences were not constant among all GM and non-GM counterparts Leaf proteomic profiles were analyzed using two-dimensional gel electrophoresis (2DE) coupled to mass spectrometry, using six 2DE gels per variety The comparison between MON810 and its counterpart was limited to qualitative differences of fully reproducible protein spot:patterns Twelve exclusive proteins were observed in two of four, maize,variety pairs, all of these leaf proteins were variety specific In this study, MON810 leaf proteomes of four varieties were similar to non-GM counterpart leaf proteomes