Automated free-solution isotachophoresis:: Instrumentation and fractionation of human serum proteins

被引：13

作者：

Böttcher, A ^{[1
]}

Möllers, C ^{[1
]}

Lackner, KJ ^{[1
]}

Schmitz, G ^{[1
]}

机构：

[1] Univ Regensburg, Inst Clin Chem & Lab Med, D-93042 Regensburg, Germany

来源：

ELECTROPHORESIS | 1998年 / 19卷 / 07期

关键词：

preparative isotachophoresis; human serum proteins;

D O I：

10.1002/elps.1150190710

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An automated free-solution isotachophoresis system (FS-ITP) for preparative fractionation of biopolymers is described, operated in a batch mode. The dimension of the separation chamber allows an up to 1200-fold higher sample load compared to separation in capillaries of 180 mu m inner diameter as used in analytical capillary isotachophoresis (C-ITP). The preparative capacity of the system is within the milligram range. The method is fully compatible with analytical C-ITP, which is essential for preparative-scale isotachophoresis with regard to optimization of electrolyte systems and the search for suitable spacers. As a model application the fractionation of human serum proteins is reported. The collected fractions were analyzed by C-ITP and agarose gel electrophoresis.

引用

页码：1110 / 1116

页数：7

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