Optical imaging of spontaneous breast tumors using protease sensing 'Smart' optical probes

被引:64
作者
Bremer, C
Ntziachristos, V
Weitkamp, B
Theilmeier, G
Heindel, W
Weissleder, R
机构
[1] Univ Munster, Dept Clin Radiol, D-48129 Munster, Germany
[2] Univ Munster, Dept Anaesthesiol & Intens Care Med, D-48129 Munster, Germany
[3] Univ Munster, Inst Anat, D-48129 Munster, Germany
[4] Massachusetts Gen Hosp, Ctr Mol Imaging Res, Charlestown, MA USA
[5] Univ Munster, Interdisciplinary Ctr Clin Res, D-4400 Munster, Germany
关键词
optical imaging; breast cancer; proteases; cathepsin; molecular imaging;
D O I
10.1097/01.rli.0000163797.23172.90
中图分类号
R8 [特种医学]; R445 [影像诊断学];
学科分类号
1002 ; 100207 ; 1009 ;
摘要
Objective: The objective of this study was to determine if spontaneous breast cancer lesions can be detected by fluorescence reflectance imaging (FRI) and fluorescence mediated tomography (FMT) using protease-sensing optical probes. Materials and Methods: Transgenic (FVB/N-TgN (WapHRAS)-69Lin y(SJL))) mice, which spontaneously develop breast cancer, were injected intravenously with a cathepsin-sensing fluorescent imaging probe. FRI and EMT were performed 24 hours after probe injection and region of interest (ROI) analysis was performed. Magnetic resonance images were acquired for anatomic coregistration with the FMT data. Moreover, correlative immunohistochemistry and fluorescence microscopy were performed. Results: All tumor nodules were clearly delineated by FRI showing an average signal intensity of 380 +/- 106 AU. Similarly, tumors were clearly detected by FMT imaging. Immunohistochemistry confirmed cathepsin-B expression of primary tumors and fluorescence microscopy revealed a strong Cy 5.5 deposition in the tissue. Conclusions: FRI and FMT using "smart" protease sensing probes pen-nits detection of experimental spontaneous breast cancers. Because the expression levels of various proteases correlate with patient outcome, this technique may not only help to detect, but also to differentiate breast cancers noninvasively.
引用
收藏
页码:321 / 327
页数:7
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