DNA Extraction Columns Contaminated with Murine Sequences

被引：58

作者：

Erlwein, Otto ^{[1
]}

Robinson, Mark J. ^{[1
]}

Dustan, Simon ^{[1
]}

Weber, Jonathan ^{[1
]}

Kaye, Steve ^{[1
]}

McClure, Myra O. ^{[1
]}

机构：

[1] Univ London Imperial Coll Sci Technol & Med, Infect Dis Sect, Jefferiss Res Trust Labs, London, England

来源：

PLOS ONE | 2011年 / 6卷 / 08期

关键词：

VIRUS-RELATED VIRUS; CHRONIC-FATIGUE-SYNDROME; PROSTATE-CANCER; RETROVIRUS XMRV; MOUSE DNA; ABSENCE; PREVALENCE; FAILURE; SAMPLES; TUMORS;

D O I：

10.1371/journal.pone.0023484

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Sequences of the novel gammaretrovirus, xenotropic murine leukemia virus-related virus (XMRV) have been described in human prostate cancer tissue, although the amounts of DNA are low. Furthermore, XMRV sequences and polytropic (p) murine leukemia viruses (MLVs) have been reported in patients with chronic fatigue syndrome (CFS). In assessing the prevalence of XMRV in prostate cancer tissue samples we discovered that eluates from naive DNA purification columns, when subjected to PCR with primers designed to detect genomic mouse DNA contamination, occasionally gave rise to amplification products. Further PCR analysis, using primers to detect XMRV, revealed sequences derived from XMRV and pMLVs from mouse and human DNA and DNA of unspecified origin. Thus, DNA purification columns can present problems when used to detect minute amounts of DNA targets by highly sensitive amplification techniques.

引用

页数：4

共 24 条

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