Three-dimensional sub-100 nm resolution fluorescence microscopy of thick samples

被引：591

作者：

Juette, Manuel F. ^{[1
,5
,6
]}

Gould, Travis J. ^{[2
,3
]}

Lessard, Mark D. ^{[1
]}

Mlodzianoski, Michael J. ^{[1
]}

Nagpure, Bhupendra S. ^{[1
,3
]}

Bennett, Brian T. ^{[4
]}

Hess, Samuel T. ^{[2
,3
]}

Bewersdorf, Joerg ^{[1
]}

机构：

[1] Jackson Lab, Inst Mol Biophys, Bar Harbor, ME 04609 USA

[2] Univ Maine, Inst Mol Biophys, Orono, ME 04469 USA

[3] Univ Maine, Dept Phys & Astron, Orono, ME 04469 USA

[4] Active Motif Inc, Carlsbad, CA 92008 USA

[5] Max Planck Inst Met Res, Dept New Mat & Biosyst, D-70569 Stuttgart, Germany

[6] Heidelberg Univ, Dept Biophys Chem, D-70569 Stuttgart, Germany

来源：

NATURE METHODS | 2008年 / 5卷 / 06期

关键词：

D O I：

10.1038/nmeth.1211

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Imaging volumes as thick as whole cells at three-dimensional (3D) super-resolution is required to reveal unknown features of cellular organization. We report a light microscope that generates images with translationally invariant 30 x 30 x 75 nm resolution over a depth of several micrometers. This method, named biplane (BP) FPALM, combines a double-plane detection scheme with fluorescence photoactivation localization microscopy (FPALM) enabling 3D sub-diffraction resolution without compromising speed or sensitivity.

引用

页码：527 / 529

页数：3

共 15 条

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