Partial characterization and immunomodulatory activity of polysaccharides from the stem of Dendrobium officinale (Tiepishihu) in vitro

被引：322

作者：

Xia, Linjing ^{[1
,2
]}

Liu, Xiaofei ^{[1
,3
]}

Guo, Huiyuan ^{[1
,2
]}

Zhang, Hao ^{[1
,2
]}

Zhu, Jun ^{[1
,2
]}

Ren, Fazheng ^{[1
,2
]}

机构：

[1] China Agr Univ, Coll Food Sci & Nutr Engn, Beijing 100083, Peoples R China

[2] Minist Educ, Key Lab Funct Dairy, Beijing 100083, Peoples R China

[3] Beijing Higher Inst Engn Res Ctr Anim Prod, Beijing 100083, Peoples R China

来源：

JOURNAL OF FUNCTIONAL FOODS | 2012年 / 4卷 / 01期

关键词：

Dendrobium officinale; Polysaccharide; Characterization; Immunomodulatory activity; PERITONEAL-MACROPHAGES; RECOGNITION; ANTITUMOR; CELLS; MICE;

D O I：

10.1016/j.jff.2011.12.006

中图分类号：

TS2 [食品工业];

学科分类号：

100403 [营养与食品卫生学];

摘要：

The crude polysaccharide from the stem of Dendrobium officinale (DOP) was obtained by water extraction and ethanol precipitation. Two fractions of DOP, DOP-1 and DOP-2, were fractioned by DEAE cellulose and Sephacryl S-400 gel filtration column chromatography. Their molecular weights and monosaccharide compositions were studied. The immunomodulatory activity of DOP, DOP-1 and DOP-2 was evaluated with in vitro cell models. The results showed that the 533.7 kDa DOP-1 and 159.5 kDa DOP-2 were mainly composed of mannose and glucose. Both DOP and its purified fractions significantly (P < 0.05) promoted splenocyte proliferation, enhanced natural killer cell-mediated cytotoxicity, and increased the phagocytosis and nitric oxide production of macrophages. Moreover, these two fractions caused a significant (P < 0.05) stimulation of cytokine secretion of both splenocytes and macrophages. These findings suggest that Dendrobium officinale polysaccharides should be explored as novel potential immunomodulators. (C) 2012 Published by Elsevier Ltd.

引用

页码：294 / 301

页数：8

共 35 条

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